Suhaini Sudi and Nurfazlin Ismail and Siti Nureza Lahaba and Nur Atikah Anuar and Wenne Junarsi Rozana William and Siti Yasmin Mohd Ikmal Hisham and Syarifah A'eesyah Syed Shikh and Goh, Lucky Poh Wah and Eleanor Joanne Muyou and Caroline Sunggip (2024) Targeted anti-cancer effects of Octomeles sumatrana on colorectal cancer cells.
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Abstract
Colorectal cancer (CRC) continues to be a significant public health concern due to its high incidence and mortality rates. The exploration of bioactive natural compounds as adjuncts to traditional chemotherapeutic agents holds promise for improving treatment outcomes against CRC. This study investigates the selective anti-cancer properties of methanolic extract of Octomeles sumatrana (OS) on CRC cell line HCT15 and normal colon cell line CCD112, focusing on its anti-proliferative, antimigration, and anti-oxidative effects. Cytotoxicity was assessed using the MTT assay, revealing OS IC50 values of 8.37±0.92 μg/ml for HCT15 and 121.30±2.09 μg/ml for CCD112 cells. Comparatively, the control drug doxorubicin (Dox) exhibited IC50 values of 20.98±1.32 μg/ml for HCT15 and 6.14±0.79 μg/ml for CCD112, highlighting OS's superior selectivity (selectivity index: 14.5 for OS vs. 0.29 for Dox). OS significantly reduced the migration rate of HCT15 cells to 1.33% compared to 22.33% in untreated controls (p<0.05). Furthermore, intracellular superoxide detection using DHE dye indicated a marked reduction in oxidative stress in OS-treated HCT15 cells (p<0.05). These results suggest that OS selectively inhibits cancer cell proliferation, mitigates migration, and reduces oxidative stress, showing promise as an ethnomedicinal candidate for CRC treatment. The study emphasizes OS’s potential as a natural adjunct or alternative to conventional therapies, warranting further research into its bioactive compounds and mechanisms of action.
| Item Type: | Proceedings |
|---|---|
| Keyword: | Octomeles sumatrana, colorectal cancer, anti-proliferative, anti-migration, antioxidant |
| Subjects: | Q Science > QH Natural history > QH1-278.5 Natural history (General) S Agriculture > SB Plant culture > SB1-1110 Plant culture |
| Department: | FACULTY > Faculty of Science and Natural Resources |
| Depositing User: | SITI AZIZAH BINTI IDRIS - |
| Date Deposited: | 18 Mar 2025 14:21 |
| Last Modified: | 18 Mar 2025 14:21 |
| URI: | https://eprints.ums.edu.my/id/eprint/43239 |
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