Wong, Wei Ting (2014) Antibacterial compounds of filamentous fungi isolated from termite mounds of macrotermes gilvus hagen. Masters thesis, Universiti Malaysia Sabah.
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Abstract
As a baseline study, this research is an initial attempt to: 1) enumerate actinomycetes and filamentous fungi from inner soil and fungus comb in termite mounds of Macrotermes gilvus Hagen; 2) screen and identify bioactive actinomycetes and filamentous fungi against human pathogenic bacteria; 3) perform chemical profiling for bioactive actinomycetes and filamentous fungi and 4) isolate and elucidate chemical structures of bioactive compound of selected actinomycetes and filamentous fungi. A total of five termite mounds of Macrotermes gilvus Hagen were randomly sampled for its inner soil and fungus comb from Borneo Samudera oil palm plantation, Kota Marudu, Kota Kinabalu, Sabah. Forty-two strains of actinomycetes and forteen strains of filamentous fungi were found enumerated on potato dextrose agar (PDA) and actinomycetes isolation agar (AIA) upon 106 serial dilution. Upon cut-and-put bioassay screening against human pathogenic bacteria, 3 selected bioactive strains were recorded by using image analyser and scanning electron microscope (SEM). Each strains were small scale batch culture in potato dextrose broth (PDB) with a suspension of 1 x 106 mL/ spores. Filtered mycelium from liquid broth was dried in oven at 50ºC for 2 days. Dried mycelium and broth were ethyl acetate (EtoAc) extracted and later distinctly succesive extracted with methanol and buthanol respectively. To obtain biomass, scale up broth culture was performed for selected strains after crude extract screening against human pathogenic bacteria. Upon the state-of-the-art of separation of preparative thin layer chromatography (PTLC), 4 compounds namely: compound A, B, C and D were isolated. Based on the data collected from nuclear magnetic resonance (NMR), optical rotation, and a comparison to published scientific papers, Compounds B, C and D were identified as Benzoic acid, Vanilic acid, and Ergosterol peroxide. While, compound A was an unknown compounds which its chemical structure was verified by a high resolution iron trap time of flight mass spectroscopic (HRITTOF MS) to be correct as exact mass was shown to be 378.3287 (mmu: -1.0). Thus, this research suggested termite mound in Sabah as a new source for providing bioactive compounds of actinomycetes and filamentous fungi.
| Item Type: | Thesis (Masters) |
|---|---|
| Keyword: | Actinomycetes, Filamentous fungi, Termite mounds, Macrotermes gilvus hagen, Bioactive compounds, Human pathogenic bacteria, Chemical profiling |
| Subjects: | Q Science > QH Natural history > QH301-705.5 Biology (General) > QH540-549.5 Ecology |
| Department: | INSTITUTE > Institute for Tropical Biology and Conservation |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 09 Apr 2025 10:06 |
| Last Modified: | 09 Apr 2025 10:06 |
| URI: | https://eprints.ums.edu.my/id/eprint/43365 |
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