Mechanistic insights into the hepatoprotective properties of Mallotus phillipensis fruit: targeting proinflammatory cytokines and free radicals using isolated compound

Waseem Rizvi and Syed Shariq Naeem and Ompal Singh and Shagufta Moin and Kafil Akhtar and Syed Najmul Hejaz Azmi and Zubair Ahmed Memon and Monowarul Mobin Siddique and Zainul Amiruddin Zakaria and Qamar Uddin Ahmed (2024) Mechanistic insights into the hepatoprotective properties of Mallotus phillipensis fruit: targeting proinflammatory cytokines and free radicals using isolated compound. Journal of King Saud University - Science, 36. pp. 1-9. ISSN 1018-3647

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Abstract

Ethnopharmacological relevance: Mallotus phillipensis (MP) belongs to the endangered plants of great medicinal value. Widely used in the Ayurvedic healthcare system, parts of the plant which are rich in secondary metabolites are used in the treatment of several diseases like eczema, bronchitis, worm infestation, diabetes, kidney stones, cancer, and malaria. In ethnomedicine, MP has been reported to be traditionally used in the treatment and healing of jaundice and inflammatory conditions in the Ayurveda system. This plant was thus selected to explore its hepatoprotective activity. Objective: This study was undertaken to investigate the protective property of MP and a flavanone i.e. 7,4′- Dihydroxy-3′′,3′′-dimethyl-(5,6-pyrano-2′′-one)-8′′′-(3′′′,3′′′-dimethylallyl)-flavanone (A4) previously identified and obtained from the same plant on CCl4-induced liver injury in animal models to validate the folkloric claims. Traditional medicinal plants have the potential to provide phytoconstituents capable of healing liver cells. In this regard, MP is used in various traditional medicinal systems to treat several diseases. Though it has been studied for its hepatoprotective potential, activity on the isolated compounds is yet to be reported to confirm the active principle. Therefore, the study assessed the hepatoprotective effect of ethyl acetate and methanol extracts and a flavanone isolated from MP fruit extract. Methods: Methanol and ethyl acetate fractions of MP fruits at two different doses (300 mg/kg and 500 mg/kg) and a flavanone (A4-50 mg/kg) isolated from MP were tested for hepatoprotective potential in rats. At 50 mg/kg dosage/ day orally, silymarin was used as a standard drug. The effect on liver enzymes and serum cytokines was also verified in animal models. Histopathology and antioxidant tests were performed on liver tissue. Elemental analysis, 1 H- & 13C NMR, MS, IR and UV spectral data were used to characterize the structure of isolated flavanone (A4).

Item Type:	Article
Keyword:	Hepatoprotection Mallotus phillipensis Cytokine Isolated compound Flavanone
Subjects:	Q Science > Q Science (General) > Q1-390 Science (General) Q Science > QK Botany > QK1-989 Botany > QK474.8-495 Spermatophyta. Phanerogams > QK495 Angiosperms
Department:	FACULTY > Faculty of Medicine and Health Sciences
Depositing User:	ABDULLAH BIN SABUDIN -
Date Deposited:	30 Apr 2025 15:36
Last Modified:	30 Apr 2025 15:36
URI:	https://eprints.ums.edu.my/id/eprint/43686

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