Isolation and identification of cadmium-reducing bacteria from contaminated coastal sediment in the Northern Coast of Indramayu, Indonesia

Tri Dewi Kusumaningrum Pribadi and Buntora Pasaribu and Kalysta Fellatami and Jiang Mingguo and Azimah Ismail and Choerunnisa Febriani and Ching Fui Fui @ Faihana Ching Abdullah and Yudi Nurul Ihsan (2025) Isolation and identification of cadmium-reducing bacteria from contaminated coastal sediment in the Northern Coast of Indramayu, Indonesia. Pakistan Journal of Biological Sciences, 28. pp. 102-110. ISSN 1028-8880

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Abstract

Background and Objective: Cadmium (Cd) is one of the heavy metal pollutants and its accumulation impacts the sustainability of marine organisms. Current research aimed to isolate and identify the cadmium-reducing bacteria from contaminated coastal sediment in Karangsong Port, Indramayu, Indonesia. The isolates were investigated for their potential to reduce cadmium and showed the cadmium reduction drastically up to 50% at 6 hrs treated under different cadmium concentrations of 0, 5, 1 and 1.5 ppm, respectively. Materials and Methods: Morphological characteristics were observed in most of the isolates. Out of 8 isolates, two selected strains such as Karangsong Cd 3 and Cd 7 were identified by 16S rRNA sequencing as Pseudoalteromonas issachenkonii strain KMM 3549 (Acc. No. NR 025139.1) and Pseudoalteromonas tetraodonis GFC strain IAM 14160 (Acc. No. NR 041787.1), respectively. Results: The cadmium resistance profile showed that the selected isolates were resistant to various concentrations of cadmium (Cd). The isolates reduced the concentration of cadmium drastically up to 50% at 6 hrs. The results demonstrated the two bacteria are possible to remove the cadmium from seawater containing cadmium. The gram staining showed bacterial colony morphology were diplobacilli and coccobacillus. Conclusion: These results suggested that the Karangsong Cd 3 and Cd 7 could facilitate the new references of future microbial applications for bioremediation efforts.

Item Type:	Article
Keyword:	Pseudoalteromonas sp., contamination, bioremediation, heavy metal, cadmium
Subjects:	Q Science > QR Microbiology > QR1-502 Microbiology > QR75-99.5 Bacteria T Technology > TD Environmental technology. Sanitary engineering > TD1-1066 Environmental technology. Sanitary engineering
Department:	INSTITUTE > Borneo Marine Research Institute
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	10 Jun 2025 11:27
Last Modified:	10 Jun 2025 11:27
URI:	https://eprints.ums.edu.my/id/eprint/43972

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