Chenghao Chu and Bin Liu and Yongwei Zhang and Zhangxuan Xu and Bin Wang and Kai Ling Chin (2024) Microrna-668-3p mediates macrophage m2 polarization by targeting nfkbia to affect gastric cancer cell proliferation and migration. Tohoku Journal of Experimental Medicine. pp. 1-25.
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Abstract
Macrophage polarization is implicated in the pathological mechanism of gastric cancer (GC). This study investigated how the miR-668-3p/ Nuclear factor kappa B inhibitor alpha (NFKBIA) axis drives macrophage polarization to contribute to GC progression. Inhibitors or shRNA were used to interfere with the expression of miR-668-3p or NFKBIA in the GC cell line. Subsequently, CCK-8, EdU, wound healing, and transwell assays were used to assess the biological behavior of the GC cells. Bioinformatics analysis predicted the target connection between miR-668-3p and NFKBIA, and a dual luciferase reporter gene experiment confirmed this relationship. After THP-1 macrophages were co-cultured with the supernatant of transfected GC cells, the M1 and M2 macrophage phenotypes were determined. Subsequently, these THP-1 macrophages were co-cultured with GC cells using the Transwell, and the biological behaviors of the GC cells were determiend. miR-668-3p inhibitor suppressed proliferation, invasion and migration of GC cells. The phenotype of M1 macrophage (IL-1β, TNF-α and IL-6) was boosted yet the phenotype of M2 macrophage (CD206, Fizz1 and IL-10) was declined by miR-668-3p inhibitor. NFKBIA was the target gene of miR-668-3p and it reversed the effects of miR-668-3p inhibitor on macrophage polarization and biological behaviors of the GC cells.miR-668-3p suppressed NFKBIA in GC cells to mediate M2 polarization of macrophages, thereby facilitating the tumorigenesis of GC. Fullsize Image
| Item Type: | Article |
|---|---|
| Keyword: | Gastric cancer; macrophage polarization; miR-668-3p; NFKBIA; metastasis |
| Subjects: | R Medicine > RC Internal medicine > RC31-1245 Internal medicine > RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens R Medicine > RC Internal medicine > RC31-1245 Internal medicine > RC581-951 Specialties of internal medicine > RC799-869 Diseases of the digestive system. Gastroenterology |
| Department: | FACULTY > Faculty of Medicine and Health Sciences |
| Depositing User: | ABDULLAH BIN SABUDIN - |
| Date Deposited: | 10 Jun 2025 11:46 |
| Last Modified: | 10 Jun 2025 11:46 |
| URI: | https://eprints.ums.edu.my/id/eprint/44000 |
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