Imelda Geoffrey (2018) Optimization of lipid production for biodiesel feedstock by rhodotorula sp. isolated from Mamut copper mine, Sabah. Masters thesis, Universiti Malaysia Sabah.
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Abstract
Biodiesel is one of the environmentally benign fuel due to its unique properties that are biodegradability, renewability and non-toxicity. The third generation of biodiesel using oleaginous microbes is capable to become the new source of biodiesel. Previous studies show that yeast Rhodotorula sp. have potential in producing lipid for biodiesel feedstock at certain conditions. Environmental stress like nutrients depletion especially nitrogen is associated with high lipid content. Understanding the lipid metabolism process and the species that has high lipid accumulation is very essential. This study was carried out using Rhodotorula sp. isolated from Mamut Copper Mine, Sabah. The aims of this study is to identify the optimum cultivation condition of Rhodotorula sp. yeast for high lipid production, including temperature, pH and ratio of nitrogen and carbon and to identify the species of Rhodotorula sp. based on Dl and D2 region of the large subunit ribosomal DNA. The experimental design for the optimization process was done by applying Response Surface Methodology (RSM). The extraction of lipid was determined using methanol: chloroform: water (1: 1: 1) lipid extraction method . Also, lipid yield was analysed according to the relationship between selected parameters. The results showed the best conditions in optimum oil production is at pH 5.62, temperature 27.68°C and carbon to nitrogen ratio 107.29. In addition, the results of the ribosomal DNA sequence analysis revealed that the yeast species was identified as Rhodotorula toruloides, showing 99% similarity and was supported with finding from phylogenetic tree construction. The finding of this study is useful for further studies in microbial-oil production.
| Item Type: | Thesis (Masters) |
|---|---|
| Keyword: | Biodiesel, Microbial oil, Third-generation biofuels, Rhodotorula sp. |
| Subjects: | T Technology > TP Chemical technology > TP1-1185 Chemical technology > TP315-360 Fuel |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 13 Jun 2025 15:00 |
| Last Modified: | 13 Jun 2025 15:00 |
| URI: | https://eprints.ums.edu.my/id/eprint/44041 |
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