Enhancement and cryostorage of Leonurus sibiricus L. seeds

Jovinia Jowinis (2011) Enhancement and cryostorage of Leonurus sibiricus L. seeds. Masters thesis, Universiti Malaysia Sabah.

	Text ABSTRACT.pdf Download (235kB)
	Text FULLTEXT..pdf Restricted to Registered users only Download (7MB)

Abstract

Malaysian seed production face constraints due to high humidity and temperature which leads to the transmission of pests and diseases. Improvements in breeding programme is needed for a dynamic seed industry. This research investigate the storability of Leonurus sibiricus L. seeds in typical storage and the possibility of cryostorage. Other than that, it also look into priming treatments for better germination of the seeds. Leonurus sibiricus L. is a famous herb of Sarawak. Seeds were collected from the Agriculture Research Centre in Kuching, Sarawak. The effect of five different environments for the basic seed storage; ambient room (28 - 30°C), air-conditioned room (22-24°C), refrigerator (3-5°C), incubator (40°C) and freezer (0 - -4°C) and cryopreservation techniques were assessed on the storage life of Leonurus sibiricus L. seeds for better breeding programme. Cryopreservation techniques conducted were vitrification and dehydration. Enhancement treatments were also conducted to assess the response of the seeds to priming ( osmopriming and hydropriming) and dehydration (silica gel and sucrose). Storage in refrigerator gave the highest germination percentage which is 32%. Vitrification technique has maintained about 44% germination at 10 minutes exposure to Plant Vitrification Solution 2 (PVS2). But Linear Regression analysis showed that seeds exposed 50 minutes to PVS2 solution can be kept longer. Dehydration has maintained about the 44% after 32 days in storage at below 10% seeds moisture content. Hydropriming at 30°C and 35°C resulting the highest germination, which is 55%. Osmoconditioning with O. lM Polyethylene Glycol 6000 solution at 35°C for 10 hours produced more seedlings as compared to other concentrations. Sucrose dehydration has enhanced the seed germination percentage to 58% after 1 hour of exposure to 0.8M sucrose solution. Silica gel dehydration resulted in 25% germination and maintained at 3% moisture content by the end of 18th day of storage. Storing in refrigerator gave the highest percentage but cryopreservation is also promising for storage of L. sibiricus seeds.

Item Type:	Thesis (Masters)
Keyword:	Leonurus sibiricus, Storage, Vitrification, Dehydration, Osmopriming, Hydropriming
Subjects:	S Agriculture > SB Plant culture > SB1-1110 Plant culture > SB113.2-118.46 Seeds. Seed technology
Department:	INSTITUTE > Institute for Tropical Biology and Conservation
Depositing User:	DG MASNIAH AHMAD -
Date Deposited:	17 Jun 2025 11:03
Last Modified:	17 Jun 2025 11:03
URI:	https://eprints.ums.edu.my/id/eprint/44098

Actions (login required)

View Item