Caysandra Audrey Michelle Elwon (2016) Hypoglycemic and antioxidative effects of herbal plant against alloxan induced diabetes mellitus in rats. Masters thesis, Universiti Malaysia Sabah.
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Abstract
Diabetes mellitus is a serious endocrine disorder that causes millions of death worldwide. However, currently only insulin and oral hypoglycaemic drugs are used to treat diabetes. Herbal medicines have been highly esteemed source of medicine throughout the human history. Thus, the purpose of this study was to investigate the effect of herbal plants against alloxan induced diabetes mellitus and oxidative stress in rats. Phytochemical constituents and antioxidant activity of Donax grandis and O/denlandia corymbosa were evaluated. Aqueous 0. corymbosa and D. grandis extracts were tested for phytochemical screening, total phenolic, flavonoids content and DPPH free radical scavenging activity. Phytochemical screening revealed the presence of flavonoids, saponins, and tannin in both plants. Flavonoids, saponin and tannin have been reported in previous studies to have anti-diabetic properties. As 0. corymbosa had been shown to protect against various kinds of diseases involving oxidative stress, it is possible that pre-treatment and treatment of animals with 0. corymbosa may attenuate alloxan induced diabetes in rats. Treatment of rats with alloxan resulted in a significant increase (P<0.05) level of blood glucose. On the other hand, oxidative stress was noticed in pancreatic tissue as evidenced by a significant decrease in glutathione level, catalase activity, and also significant increase in malondialdehyde when compared to normal saline control group. Pancreases were examined by haematoxylin and eosin staining. Additionally, oxidative stress markers were consistent with the pancreatic histopathological studies. Treatment of diabetic rats with 0. corymbosa extract significantly prevented these alterations and attenuated alloxan-induced oxidative stress. The results of the present study indicated that the hypoglycemic potentials of 0. corymbosa might be ascribable to its antioxidant and free radical scavenging properties. Thus, it concluded that 0. corymbosa may be helpful in the prevention of diabetic complications associated with oxidative stress.
| Item Type: | Thesis (Masters) |
|---|---|
| Keyword: | Oldenlandia corymbosa, Donax grandis, Diabetes mellitus, Oxidative stress, Phytochemicals, Antioxidants, Alloxan, Rat model |
| Subjects: | R Medicine > RC Internal medicine > RC31-1245 Internal medicine > RC581-951 Specialties of internal medicine > RC648-665 Diseases of the endocrine glands. Clinical endocrinology |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 17 Jun 2025 11:18 |
| Last Modified: | 17 Jun 2025 11:18 |
| URI: | https://eprints.ums.edu.my/id/eprint/44113 |
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