Dk. Noor Rashidah Pg. Mohd. Noor (2016) The effect of squalene and lupeol on human breast cancer cell line, MCF-7. Masters thesis, Universiti Malaysia Sabah.
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Abstract
Breast cancer is the most cancer affected among women worldwide. Although there have been many available drugs to treat and prevent cancer, the statistics is still alarming. Moreover, there have been studies showing the long-term effect of current cancer drugs. Squalene and lupeol are natural substances that can be found in plants. Many benefits of both compounds have been discovered and recently theirs properties of preventing breast cancer. Therefore, the aim of this research was to study the anticancer effect of squalene and lupeol on human breast cancer cell line. The ability of squalene and lupeol to inhibit the proliferation of MCF-7 cells was conducted by performing MTT assay. The results of the MTT assay were used to determine the IC50 of the squalene and lupeol in inhibiting 50% of cancer cells' population. The formation of DNA laddering on agarose gel was perfomed to detect any apoptosis that can cause fragmentation of DNA of MCF-7. MCF-7 cells were also stained with methylene blue to observe the morphology changes after treatment. Based on the results obtained, IC50 only achieved for lupeol (ICso = Bμg/ml) after treatment but not after squalene treatment. Same goes for the staining, lupeol caused the morphological changes of MCF-7 cells change but not for squalene. In conclusion, these results suggest that lupeol inhibits the growth of MCF-7 and potentially induce cell death through apoptosis based the formation of DNA laddering on agarose gel after cells treated with lupeol. In contrast, there is no inhibition of squalene on MCF-7 cells. Besides that, DNA laddering was observed in cells treated with lupeol although the result was not very clear compared to positive control. Further studies are necessary to elucidate the potential used of lupeol in cancer therapeutics and especially in cancer prevention.
| Item Type: | Thesis (Masters) |
|---|---|
| Keyword: | Breast cancer, MCF-7 cell line, Lupeol, Squalene, Anticancer phytochemicals |
| Subjects: | R Medicine > RC Internal medicine > RC31-1245 Internal medicine > RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 17 Jun 2025 11:03 |
| Last Modified: | 17 Jun 2025 11:03 |
| URI: | https://eprints.ums.edu.my/id/eprint/44121 |
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