Chung, Geoffrey Wei Jei (2014) Polymorphisms of partial merozoite surface protein-1 (MSP-1) in plasmodium species isolated from Sabah. Masters thesis, Universiti Malaysia Sabah.
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Abstract
Malaria is a mosquito-borne infectious disease caused by parasitic Plasmodium species and resistance to anti-malarial medicines has been documented. Therefore, it is vital to develop malaria vaccine. One of the vaccine candidate antigens is merozoite surface protein-! (MSP-1). This study was carried out to determine the genetic diversity of msp-1 gene especially C-terminal region of Plasmodium fa/ciparum, vivax and knowlesi in Sabah. Genomic DNA was extracted from malaria infected blood samples and species-specific nested PCR was carried out to identify Plasmodium species. Three independent PCR were used to amplify the msp-1 gene of the three Plasmodium species. Gel purification of PCR product was performed prior to sequencing. Block 2 and C-terminal region of MSP-1 of ten P. fa/ciparum isolates were sequenced. Six P. knowlesi isolates and three P. vivax isolates were sequenced for C-terminal region of MSP-1. Statistical analysis was carried out in (terminal MSP-119 region for P. fa/ciparum, P. vivax and P. know/es,: Allelic variation of block 2 of Pfmsp-1 of Sabah isolates also been characterized and indicated that all Pfmsp-1 isolates are consists of MAD20-type strain. The allelic variation of Pfmsp-1 of Sabah isolates were E-TSR-L and Q-KNG-F. By comparing the rate of non-synonymous versus synonymous substitutions, the analytical results showed that MSP-lig of P. falciparum and P. knowlesi is under positive natural selection while purifying selection is acting on MSP-lig of P. vivax. No new allelic variation of P. fa/ciparum MSP-119 was observed among the Sabah isolates as the two allelic variants of Sabah isolates were previously reported. In conclusion, these results have provided a general view of polymorphism and allelic variation for P. falciparum in Sabah and complement with the existing database on the Pfmsp-119. Further studies are required to determine the allelic variation of P. vivax and P. knowlesi.
| Item Type: | Thesis (Masters) |
|---|---|
| Keyword: | Malaria, Plasmodium falciparum, Plasmodium vivax, Plasmodium knowlesi, Msp-1 |
| Subjects: | Q Science > QR Microbiology > QR1-502 Microbiology > QR180-189.5 Immunology |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 19 Jun 2025 15:42 |
| Last Modified: | 19 Jun 2025 15:42 |
| URI: | https://eprints.ums.edu.my/id/eprint/44185 |
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