Grace P. Kaling (2014) The effect of Clinacanthus nutans root extracts on the expression of apoptotic genes. Masters thesis, Universiti Malaysia Sabah.
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Abstract
Breast cancer is one of the most common cancers worldwide and the leading cause of cancer death in women. Currently, increasing number of patients has developed resistance toward anticancer drug. Hence, it is necessary to discover more effective yet less toxic anticancer drugs which are achievable through studying various plants with known medicinal properties. The ability to induce apoptosis in cancer cells is the key success of plant products as anticancer agent and is a highly desirable goal of treatment strategy for cancer control. Clinacanthus nutans is a type of medicinal plants and belongs to family Acanthaceae, it has been traditionally used in treatment of various diseases. This study was carried out to investigate the ability of Clinacanthus nutans root extracts to inhibit MCF-7 breast cancer cell line growth via apoptotic pathway. Previous MTT assay showed that the growth of treated MCF-7 cancer cells with C nutans methanol and ethyl acetate root extracts were inhibited at concentrations of 35 µg/ml and 30 µg/ml respectively. Therefore, cultured MCF-7 cancer cells were treated with methanol and ethyl acetate root extracts of C nutans with their respective concentrations. DNA fragmentation assay was performed to determine the apoptotic activity of the extracts since DNA fragmentation is a biochemical characteristic of apoptotic cells. This was done by extracting DNA from the treated cells through phenol chloroform method followed by agarose gel electrophoresis. Subsequently reverse transcription PCR was performed to evaluate the expression of two apoptotic related genes which are BCL-2 and BAX. Result obtained shows absence of DNA fragmentation or laddering pattern. On the other hand, RT-PCR results revealed that the BCL-2 expression in the treated samples has been decreased while BAX expression was not altered in either control or treated sample. In conclusion, the result obtained from this study indicates that the C nutans root extract might have a potential in regulating apoptosis pathway in breast cancer cells. However, a more comprehensive study should be conducted in the future to further establish the effect of C. nutans root extracts in mediating apoptosis.
| Item Type: | Thesis (Masters) |
|---|---|
| Keyword: | Clinacanthus nutans, Apoptosis, MCF-7, Breast cancer, BCL-2, BAX, DNA fragmentation, Medicinal plants |
| Subjects: | Q Science > QH Natural history > QH301-705.5 Biology (General) > QH573-671 Cytology |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 18 Jun 2025 10:30 |
| Last Modified: | 18 Jun 2025 10:30 |
| URI: | https://eprints.ums.edu.my/id/eprint/44195 |
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