Ting, Siew Hoo (2015) In vitro propagation and photobioreactor cultivation of commercially important seaweed, kappaphycus alveraziiln Sabah. Masters thesis, Universiti Malaysia Sabah.
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Abstract
In Sabah, there wasa problemfor conventional farming of Kappaphycusalverazii, where after seasonal variations and disease infestations, there was lack of healthy seedlings for next cultivation.In vitro tissue culture techniques can solve this problemand facilitate the propagation of commercially important genotypes which are healthy and fast growing. This research was carried out to optimize the suitable conditions for the propagation of Kappaphycusalverazii. Culture media, explants weight over volume concentration, aeration activity and light illuminescence were found to be the critical factors that affect the growth of Kappaphycusalverazii. The optimum conditions for culturing seaweeds were found to be initial explants of 0.5 to 1.0 of weight in gram over 100ml media, 50 % PES media enrichment and supplemented with 2.5 g/I BAP and 1.0g/I IAA, light intensity of 6,000 lux, continuous aeration and temperature of 24 to 27°C. In order to achieve mass cultivation of seedlings, a customized airlift photobioreactor was constructed to provide the optimal culture conditions to the seaweedcultivation. The percentage ofincrease infresh culturesper day for 60 days in the photobioreactor (6.0 %(g g-1d-1)) was found to be higher than the growth rate obtained from the culturing flask ( 4.260/o(g g-1d-1)) and the cultivation in sea (5.5%(g g-1d-1)). A study involving the profiling of protein expression between sea cultivated seaweed and clonally propagated seaweed revealed that short peptides with a molecular mass of 5-10 kDA were produced in significant amounts in naturally cultivated seaweeds but stop production during the first 12 week of laboratory culture. These peptides were revealed to be in the class of Lectin by MALTIDOF method. The reappearance of this peptide after 12 weeks of cultivation may indicate that the propagules are ready to be transferred to the natural environment as they possess defence systems for adaptation to the wild. Thus, Lectins can be applied as a biomarker to monitor the quality of seaweed cultures in vitro and this biomarker can be highly beneficial to the seaweed farming industry.
| Item Type: | Thesis (Masters) |
|---|---|
| Keyword: | Kappaphycus alvarezii, Seaweed tissue culture, Photobioreactor, In vitro propagation, Lectin biomarker |
| Subjects: | Q Science > QK Botany > QK1-989 Botany |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 18 Jun 2025 10:36 |
| Last Modified: | 18 Jun 2025 10:36 |
| URI: | https://eprints.ums.edu.my/id/eprint/44205 |
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