Azalina Farina Abd Aziz (2016) An evaluation of antioxidant and antidiabetic potential of cynometra cauliflora (nam-nam, fabaceae). Masters thesis, Universiti Malaysia Sabah.
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Abstract
Oxidative damage of biomolecules is implicated in the pathogenesis of various chronic diseases including diabetes. This has led to intensive investigation aimed at reducing the extent of such oxidative injury. Cynometra cauliflora or normally known as "Nam-nam" is a native of Malaysia, grown mainly in northern peninsular Malaysia and possesses many medicinal values in treating several diseases and for health care maintenance. However, antidiabetic and antioxidative potential of C cau/iflora have not been fully investigated. Therefore, the present study was aimed to evaluate the antioxidative, hypolipidemic and hypoglycemic potentials of C. Cau/iflora extract against alloxan induced diabetes rats. Parts of Ccau/iflora were used are; young leaves, matured leaves, stems and barks. The dry sample of young leaves, matured leaves, stems and barks from C cauliflora were tested for phytochemical screening, total phenolic, flavonoids content and DPPH free radical scavenging activity. Phytochemical screening showed the presence of tannins, saponins and flavonoids in all parts of C cauliflora. Terpenoids were found in all tested parts of the plant except in bark. The constituent of cardiac glycosides was present in all tested parts of the plant except in stem. Total phenolic content of young leaves was found to be 1831.47±1.03 mg/lO0g edible portion of dried sample. The total flavonoid content of young leaves was found to be a 33.63±0.25 mg/100g edible portion of dried sample. The radical scavenging activity was determined to be the following order: young leave>matured leave> stem> bark compared with ascorbic acid using as a standard. Based on the results of in vitro studies, matured leave was selected further for in vivo studies due to a high level of total phenolics and antioxidant capacity. Treatment of rats with alloxan resulted in a significant increase (P<0.05) level of blood glucose, total cholesterol and low density lipoprotein. On the other hand, oxidative stress was noticed in pancreatic tissue as evidenced by a significant decrease in glutathione level, catalase activity, and also significant increase in malondialdehyde when compared to normal saline control group. Pancreases were examined by hematoxylin and eosin staining. Additionally, serum biochemistry and oxidative stress markers were consistent with the pancreatic histopathological studies. Treatment of diabetic rats with C. Cauliflora extract significantly prevented these alterations and attenuated alloxaninduced oxidative stress. The results of the present study indicated that the hypolipidemic and hypoglycemic potentials of C Cauliflora might be ascribable to its antioxidant and free radical scavenging properties. Thus, it concluded that C Cauliflora may be helpful in the prevention of diabetic complications associated with oxidative stress.
| Item Type: | Thesis (Masters) |
|---|---|
| Keyword: | Cynometra cauliflora, Nam-nam plant, Antioxidant activity, Hypoglycemic effect, Hypolipidemic activity |
| Subjects: | Q Science > QK Botany > QK1-989 Botany > QK1-474.5 General Including geographical distribution |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 20 Jun 2025 16:18 |
| Last Modified: | 20 Jun 2025 16:18 |
| URI: | https://eprints.ums.edu.my/id/eprint/44222 |
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