Ghafur Rahim Mustakim (2017) Toxin compositions of green mussel Perna viridis fed with toxic dinoflagellate, Pyrodinium bahamense var. compressum. Masters thesis, Universiti Malaysia Sabah.
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Abstract
Harmful algal blooms, especially Pyrodinium bahamense var. compressum (Pbc) has been the focus of research in Sabah due to its negative impacts toward aquaculture industry and human health. This type of algae can cause Paralytic Shellfish Poisoning (PSP). However, studies on acclimatization and depression periods of PSP toxin in mussels has not been done in the affair, although the mussels are highly consumed by the people of Sabah. Paralytic Shellfish Poison (PSP) toxins for this study were investigated by means of high-performance liquid chromatography with post column fluorescence derivatization (HPLC-FLD). Shellfish samples were collected from places that historically affected by this toxin; Kuala Penyu, Kota Merudu, Kota Belud and Mantanani Island. Sample was collected from June 2015 until April 2016. Three species of shellfish (Perna viridis, Geloinasp. And Anadara sp.) was collected from all sampling locations. Perna virid1:Scollected from Kuala Penyu, shown the presence of PSP toxin analogue (decarbamoyl derivatives) which is Gonyautoxin (GTX 4), with toxin content of 30µgeq/100g tissue. However, this GTX 4 need to be reconfirmed in future analysis by using Liquid Chromatography - Mass Spectrometry (LC-MS) in order to differentiate either the peak formed was imposter or not. Then, for accumulations and depuration study of PSP toxin inside the mussel, toxic dinoflagellate, Pbc was cultured in f/2 media and fed to the green mussels, Perna viridis. Initial toxin content detected from cultured Pbc was 963 fmol/cell and toxin component detected were STX, Neo STX and GTX 6. The mussel ingested more than 99% of the Pbc cells (3 x 105 cells) once at the beginning of the experiment and accumulated a maximal amount of toxin (201fmol/100 g) at 96 hours and started to decrease after 120 hours of rearing periods. Composition of the PSP toxin accumulated in the mussels obviously different from Pbc at 3 hours after the cell supplied. The variation in toxin composition derived presumably from the transformation of toxin analogues in mussels was observed after 144 hours of the rearing period. Whereas, PSP toxin component (STX, Neo STX and GTX 6) in residue water was almost same as its origin dinoflagellate. From this study, it was clearly demonstrated that toxins from Pbc take a short period of time to accumulate and depurate toxin in the mussel's tissues after the digestion process. These findings also can be used as a basic guideline for PSP cases in the future.
| Item Type: | Thesis (Masters) |
|---|---|
| Keyword: | Harmful algal blooms, Pyrodinium bahamense var. compressum, Paralytic Shellfish Poisoning, Toxin accumulation, Depuration period |
| Subjects: | Q Science > QK Botany > QK1-989 Botany > QK504-(638) Cryptogams |
| Department: | INSTITUTE > Borneo Marine Research Institute |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 03 Jul 2025 10:50 |
| Last Modified: | 03 Jul 2025 10:50 |
| URI: | https://eprints.ums.edu.my/id/eprint/44304 |
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