Modeling of fermentative polyhydroxyalkanoate production from Cerbera odollam oil using Monod-based and multi-scale kinetic models

Lim, Sook Wei and Jibrail Kansedo and Tan, Inn Shi and Jobrun Nandong and Tan, Yie Hua and Lam, Man Kee and Clarence M. Ongkudon (2025) Modeling of fermentative polyhydroxyalkanoate production from Cerbera odollam oil using Monod-based and multi-scale kinetic models. Bioresource Technology, 435. pp. 1-11. ISSN 0960-8524

Text
FULL TEXT.pdf
Restricted to Registered users only
Download (4MB) | Request a copy

URL: https://www.sciencedirect.com/science/article/pii/...

Abstract

Two mathematical models were developed to elucidate the biosynthesis of polyhydroxyalkanoates (PHAs) by Pseudomonas resinovorans DSM 21078 from Cerbera odollam oil – (1) The modified Monod-based kinetic model was integrated with Andrews, Haldane, and Logistic kinetics to describe carbon, nitrogen, and self-inhibition effects on cell growth and PHA production. An additional nitrogen regulatory factor was incorporated to account for urease activity and alternative nitrogen sources during fermentation. (2) The multi-scale kinetic model was adapted to capture three dynamic modes in the biosynthesis process (i.e., using Triple First-Order model), namely fast and slow modes of PHA production, and PHA degradation mode. The fast factors could be prompted by the de novo fatty acid synthesis pathway, saturated fatty acids in the oil compounds, and late-exponential-phase cells in the culture. Overall, the established mathematical models offered a robust predictive framework, providing valuable insights into fermentation behaviour that could advance oil-based PHA production systems.

Item Type:	Article
Keyword:	Oil-based polyhydroxyalkanoates, Pseudomonas resinovorans, Monod-based kinetics, Multi-scale kinetics, Fermentation modeling
Subjects:	Q Science > QR Microbiology > QR1-502 Microbiology > QR75-99.5 Bacteria T Technology > TP Chemical technology > TP1-1185 Chemical technology > TP248.13-248.65 Biotechnology
Department:	INSTITUTE > Biotechnology Research Institute (BRI)
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	15 Jul 2025 12:33
Last Modified:	15 Jul 2025 12:33
URI:	https://eprints.ums.edu.my/id/eprint/44451

Actions (login required)

View Item