Effect of miR‑29a‑3p on renal interstitial fibrosis in diabetic kidney disease through FOXP1‑mediated TGF‑β1/Smad3 signaling pathway

Juntai Zhang and Yan Qin and Jie Liu and Jie Ding and Mengying Xu and Li Yang and Yuanxin Zheng and Chin Kai Ling and Xi Zhang (2025) Effect of miR‑29a‑3p on renal interstitial fibrosis in diabetic kidney disease through FOXP1‑mediated TGF‑β1/Smad3 signaling pathway. Springer Nature, 77 (120). pp. 1-18. ISSN 0920-9069

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Abstract

Renal interstitial fibrosis (RIF) is a major manifestation of diabetic kidney disease (DKD). This study aimed to elucidate the specific mechanism by which miR-29a-3p affects RIF in DKD through Forkhead box protein 1 (FOXP1)-mediated TGF-β1/Smad3 and to provide novel ideas and therapeutic targets for RIF. Cell and animal models were constructed, and CCK-8, flow cytometry, Western blotting, immunofluorescence, RT‒qPCR, Mas- son’s trichrome staining, hematoxylin–eosin (HE) and periodic acid–Schiff (PAS) staining, Sirius red staining, and immunohistochemistry were used to detect the related indicators of RIF in DKD. The interaction between miR-29a-3p and FOXP1 was confirmed using a dual-luciferase assay. These findings suggested that miR-29a-3p can act on renal interstitial fibrosis cells in DKD through the TGF-β1/Smad3 signaling pathway mediated by FOXP1. The overexpression of miR-29a-3p inhibited the expression of fibrin-associated proteins in renal tissue, contracted the mesangial matrix and decreased the accumulation of renal fibrils to ultimately alleviate RIF in DKD. This study is the first to propose the specific molecular mechanism of miR-29a-3p in renal interstitial cells in DKD, which provides novel targets and strategies for the clinical treatment of RIF-related DKD.

Item Type:	Article
Keyword:	Diabetic kidney disease, miR-29a-3p, FOXP1, TGF-β1/Smad3, Renal interstitial fibrosis
Subjects:	R Medicine > RC Internal medicine > RC31-1245 Internal medicine > RC91-103 Disease due to physical and chemical agents R Medicine > RD Surgery > RD1-811 Surgery > RD680-688 Diseases of the locomotor system (Surgical treatment)
Department:	FACULTY > Faculty of Medicine and Health Sciences
Depositing User:	JUNAINE JASNI -
Date Deposited:	04 Aug 2025 16:54
Last Modified:	04 Aug 2025 16:54
URI:	https://eprints.ums.edu.my/id/eprint/44672

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