Harvesting Chaetoceros gracilis by flocculation using Chitosan

W Yamin and Sitti Raehanah Muhamad Shaleh and Ching Fui Fui @ Faihana Ching Abdullah and Rafidah Binti Othman and Bernardette Mabel Manjaji Matsumoto and Saleem Mustafa and S Shigeharu and G Kandasamy (2019) Harvesting Chaetoceros gracilis by flocculation using Chitosan. In: 248th ECS Meeting. Chicago IL, 12-16 Oktober 2025, Hilton Chicago.

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Abstract

Flocculation is a method of harvesting microalgae by separating cells from the culture medium using flocculating agents. Ferric Chloride and alum are conventional flocculants that have the disadvantages of toxic residue. In this study, chitosan, a non-toxic flocculating agent is tested on Chaetoceros gracilis to determine the effect of culture conditions (salinity and cell density) towards the flocculation efficiency (FE). Marine diatom C. gracilis is cultured using Walne’s Media for 8 days in a temperature-controlled room and illuminated for 12 hours. Four different salinities (20, 25, 30, 35 ppt), four different cell densities (0.5, 2, 4, 6 x 10 6 cells mL -1), and six chitosan concentration (0, 15, 30, 45, 60, 75 ppm) are the parameters tested in this study. Measuring cylinder test is used to measure FE using spectrophotometer (OD750). From the results, salinity and cell density affect significantly the FE of the diatom. In 20 ppt culture, the FE was 89% after 50 minutes using 75 ppm of chitosan at a density of 4 x 10 6 cells mL-1 . Growth conditions of the diatom culture have strong influences on the flocculation performances. The chitosan can be an option for non-toxic flocculants for concentrating live feeds in the aquaculture industry.

Item Type:	Conference or Workshop Item (Other)
Keyword:	Flocculation, Aquaculture industry, Locculation performances, Culture conditions
Subjects:	S Agriculture > S Agriculture (General) > S1-(972) Agriculture (General) > S403 Agricultural missions, voyages, etc. S Agriculture > S Agriculture (General) > S1-(972) Agriculture (General) > S589.75-589.76 Agriculture and the environment
Department:	INSTITUTE > Borneo Marine Research Institute
Depositing User:	JUNAINE JASNI -
Date Deposited:	08 Aug 2025 15:18
Last Modified:	08 Aug 2025 15:18
URI:	https://eprints.ums.edu.my/id/eprint/44745

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