Investigation of bacterial antigenic fragments OMPs as a potential vaccine candidate against vibriosis in TGGG

Iffah Md Daud and Rukmiyatul Rahim and Cahyo Budiman and Mohd Tamrin Lal and Zarina Amin (2024) Investigation of bacterial antigenic fragments OMPs as a potential vaccine candidate against vibriosis in TGGG. Borneo International Journal of Biotechnology (BIJB), 4. pp. 60-68.

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Abstract

Aquaculture holds a crucial position in the economies of developing nations, making significant contribution to both food security and income. Despite its importance, aquaculture encounters challenges including bacterial infections threats like vibriosis that caused by Vibrio spp. Concerns regarding antibiotic resistance have prompted a shift towards usage of vaccine as a sustainable alternative treatment method. This study focused on the development of a fusion fragment protein GADPH-OmpK as a potential vaccine candidate against vibriosis in hybrid TGGG Grouper (Epinephelus fuscoguttatus x E. lanceolatus) and the impact it has on the expression of immune genes. The fusion fragment protein was successfully overexpressed and used to intraperitoneally immunize hybrid TGGG grouper. The immunogenetic expression of Interleukin-2, Interleukin-6 and Interferon-ϒ in the results indicated that the vaccine elicits a considerable immune response in the fish, demonstrating its potential in enhancing aquaculture sustainability. Overall, this research presents a promising avenue for advancing aquaculture practices and mitigating antibiotic resistance issues in fish farming since recombinant protein vaccine offer various advantages.

Item Type:	Article
Keyword:	Aquaculture, TGGG hybrid grouper, Vaccine, Immune response
Subjects:	S Agriculture > SH Aquaculture. Fisheries. Angling > SH1-691 Aquaculture. Fisheries. Angling > SH201-399 Fisheries > SH381-385 Whaling
Department:	FACULTY > Faculty of Science and Natural Resources
Depositing User:	DG MASNIAH AHMAD -
Date Deposited:	19 Aug 2025 10:52
Last Modified:	19 Aug 2025 10:52
URI:	https://eprints.ums.edu.my/id/eprint/44979

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