Effect of long-term storage on the quality of cryopreserved sperm of the Pangasius nasutus (Bleeker, 1863)

Nurizzati Idris and Donald Torsabo and Muhammad Yazed Abduh and Ambok Bolong Abol-Munafi and Noordiyana Mat Noordin and Ivan Chong Chu Koh (2025) Effect of long-term storage on the quality of cryopreserved sperm of the Pangasius nasutus (Bleeker, 1863). Cryobiology, 119 (105219). pp. 1-8.

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Abstract

In the present study, we investigated the effects of storage for 3, 6, 9 and 12 months on cryopreserved sperm of P. nasutus, in 10 % MeOH as a cryoprotectant with 90 % 0.9 % NaCl as an extender. Sperm quality on motility, fertilization, hatching, and deformity rates were investigated using 9-months cryopreserved sperm. Determine- Tion on the effects of different sperm to egg ratios also was evaluated using 1-year cryopreserved sperm. Post- thaw motility (PTM) of cryopreserved sperm within storage period was significantly lower compared to the initial motility of fresh sperm (51.67 ± 2.4 %). However, there was no significant change of PTM during 12 months of storage (3 months, 33.3 ± 2.33 %; 6 months, 32.0 ± 2.52; 9 months, 32.67 ± 1.76; 12 months, 33.33 ± 1.67). In addition, the motility duration was unaffected by storage as there was no significant difference compared to fresh samples. Besides that, there were no significant differences in motility, fertilization and deformity rates for fresh and 9-months cryopreserved sperm except for hatching rate. Samples stored for 1 year resulted in high fertilization across all sperm to egg ratios, showing no difference compared to fresh sperm. Cryopreserved sperm exhibited a significant higher hatching rate (9.24 ± 4.68 %) at the sperm to egg ratio of 300,000:1 compared to 150,000:1 (3.44 ± 1.7 %). However, no significant difference in deformity rates for fresh and cryopreserved at sperm to egg ratio of 300,000:1. The newly developed cryopreservation protocol using methanol successfully preserved sperm quality, maintaining fertilization rates comparable to fresh sperm. This highlights its potential application in sustainable aquaculture and conservation strategies for Pangasius nasutus.

Item Type:	Article
Keyword:	Pangasius nasutus, Fertilization, Hatching, Sperm motility, Storage duration
Subjects:	Q Science > QP Physiology > QP1-(981) Physiology > QP1-345 General Including influence of the environment R Medicine > RG Gynecology and obstetrics > RG1-991 Gynecology and obstetrics > RG133-137.6 Conception. Artificial insemination. Contraception
Department:	INSTITUTE > Borneo Marine Research Institute
Depositing User:	JUNAINE JASNI -
Date Deposited:	22 Aug 2025 10:07
Last Modified:	22 Aug 2025 10:07
URI:	https://eprints.ums.edu.my/id/eprint/45046

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