Melissa Joseph James (2005) The effects of egg quality improvements on Hatchery produced tiger prawn, penaeus monodon. Masters thesis, Universiti Malaysia Sabah.
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Abstract
This thesis investigates the effect of improving the quality of intraovarian oocytes on the larvae and juveniles in tiger prawn, Penaeus monodon. These stages form the stocking material for commercial aquaculture of tiger prawn. The work was motivated by the need to curtail the mortality in hatcheries and rearing ponds on account of poor quality of eggs and larvae. Experiments designed in this research confirmed that survival and growth of tiger prawn were significantly higher when quality of egg was improved while the oocytes were still developing in the ovary of the broodstock. Selection of broodstock with intact genetic capital enabled the effect of nutrition improvement of broodstock to manifest in the production of high quality seed for aquaculture. The genetic diversity was assessed by allozyme electrophoresis. Two polymorphic loci were recorded (HK-1 and IDH-1) from ten enzyme systems tested with percentage of polymorphic loci at 8.3%. Genetic variability was comparably high as evident from the values of mean number of alleles per locus (1.2 ± 0.1) and the observed heterozygosity (0.013 ± 0.009). There was no evidence of founder effect and inbreeding in the hatchery or of a population bottleneck in the wild stock used to produce seed. Suggestions on the conservation of genetic variability in the seed production program as well as the biochemical properties during the development of the early larvae are outlined in this thesis. Nutritional management of broodstock during the course of gonad development influenced the quality of eggs as well as that of the endogenously feeding nauplius larvae. Live prey in the form of bloodworm supplied to the female broodstock resulted in increased synthesis of RNA and protein, and improvement in the RNA/DNA ratio. DNA, which is the genetic material, remained stable and appeared to be unaffected by metabolic events in the cytoplasm. The effect was more pronounced when the tiger prawn consumed bloodworm enriched with tricalcic phosphate. The effect of broodstock enrichment on eggs was overtaken by environmental factors when exogenous feeding replaced endogenous feeding. This marked a critical period in the life of the larvae as they emerged out of dependence on maternal reserves to take on a more independent existence. Differences in growth of the various early developmental stages were noticeable, with zoea allocating more nutrients for growth and mysis making more energy demands for maintenance. Details are embodied in this thesis.
| Item Type: | Thesis (Masters) |
|---|---|
| Keyword: | Penaeus monodon, Tiger prawn, Aquaculture seed production, Oocyte quality, Larval development, Juvenile growth |
| Subjects: | S Agriculture > SH Aquaculture. Fisheries. Angling > SH1-691 Aquaculture. Fisheries. Angling > SH201-399 Fisheries > SH365-380.92 Shellfish fisheries. Shellfish culture |
| Department: | INSTITUTE > Borneo Marine Research Institute |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 29 Sep 2025 09:55 |
| Last Modified: | 29 Sep 2025 09:55 |
| URI: | https://eprints.ums.edu.my/id/eprint/45219 |
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