Screening and characterization of thermostable lipases-producing bacteria from Sabah hot springs

Nurshafrina Aida Yahya and Bak Zaibah Fazal and Rafida Razali and Clemente Michael Wong Vui Ling and Zarina Amin and Thean Chor Leow and Cahyo Budiman (2025) Screening and characterization of thermostable lipases-producing bacteria from Sabah hot springs. Malaysian Journal of Microbiology, 21 (3). pp. 311-322. ISSN 2231-7538

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Abstract

This study was aimed to screen lipase-producing bacteria (LPB) from Tawau and Poring hot springs in Sabah, Malaysia, identify the best bacterium and characterize its lipolytic activity. Methodology and results: The sediment and water samples from the hot springs were used to screen for LPB using Luria-Bertani (LB) agar containing tributyrin as a screening medium at 50 °C. The screening process yielded eight LPB isolates (C1-C8) from the Poring hot spring sediment sample. The specific lipolytic activity of the crude enzymes obtained from these isolates against the p-nitrophenol palmitate substrate ranged from 0.01×10-2 U/mL (C7) to 6.90×10-2 U/mL (C5). The optimum temperature for their activity ranged from 40 °C (C6) to 75 °C (C5), indicating that they were These LPB isolates exhibited an optimum pH range of 6.0 to 8.0 and were relatively stable in the presence of metal ions, detergents, or dimethyl sulfoxide. The LPB isolate with the highest optimum temperature activity (C5) was identified based on BLAST results of 16S rRNA gene sequence as a species of Serratia with an optimum growth temperature of 65 °C. Significance and impact of study: The study confirmed that Sabah hot springs are potential sources of thermostable LPB with unique properties that may be useful in lipid-processing-related industries.

Item Type:	Article
Keyword:	Hot springs, lipases, thermostable enzymes, thermophilic bacteria
Subjects:	Q Science > QP Physiology > QP1-(981) Physiology > QP501-801 Animal biochemistry Q Science > QR Microbiology > QR1-502 Microbiology > QR75-99.5 Bacteria
Department:	INSTITUTE > Borneo Institute for Indigenous Studies
Depositing User:	JUNAINE JASNI -
Date Deposited:	31 Oct 2025 15:40
Last Modified:	31 Oct 2025 15:40
URI:	https://eprints.ums.edu.my/id/eprint/45544

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