Whole-genome sequencing of Proteus mirabilis strain CM018 isolated from retail chicken meat

Tan, H.K and Nurzafirah Mazlan and Rinai k and Thung, T.Y (2025) Whole-genome sequencing of Proteus mirabilis strain CM018 isolated from retail chicken meat. Letters in Food Research, 01 (3). pp. 1-5.

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Abstract

In this study, Proteus mirabilis strain CM018 was isolated from retail chicken meat and confirmed by 16S rRNA gene sequencing. Whole-genome sequencing (WGS) was performed using a combination of Illumina and Oxford Nanopore Technologies (ONT), followed by hybrid assembly and polishing tools. The genome has 3,812,096 bp and 3342 protein coding sequences with GC content of 38.6%. Antibiotic resistance genes (ARGs) such as Tet(J), Cata, crp, and 3 CRISPR arrays were found in the genome. Additionally, antiviral defense systems of Gabija, PARIS, PD-Lamda-5 and Septu were detected using the webserver PADLOC and Defense Finder. Further, experimental results showed that a siphoviridae prophage was isolated using mitomycin C induction. The findings contribute to the understanding of P. mirabilis in retail chicken meat and its potential to pose a substantial public health threat.

Item Type:	Article
Keyword:	Chicken meat, Proteus mirabilis, Whole-genome sequencing, Virulence genes, Antibiotic resistance genes, Antiviral defense systems
Subjects:	Q Science > QR Microbiology > QR1-502 Microbiology > QR100-130 Microbial ecology T Technology > TX Home economics > TX1-1110 Home economics > TX642-840 Cooking
Department:	INSTITUTE > Borneo Marine Research Institute
Depositing User:	JUNAINE JASNI -
Date Deposited:	11 Nov 2025 11:51
Last Modified:	11 Nov 2025 11:51
URI:	https://eprints.ums.edu.my/id/eprint/45689

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