Fung, Joseph (2010) Production of hybrid Orchid: in vitro seed germination of Renanthera bella x Vanda helvola; Paraphalaenopsis denevei x Phalaenopsis bellina; And Vanda dearei x Vanda helvola. Universiti Malaysia Sabah. (Unpublished)
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Abstract
A hybrid is a result of the union, whether naturally occurring or man made, of two plants of different genetic backgrounds. Assisted cross-pollination was carried out by placing the pollen of one orchid plant onto the stigma of another orchid plant, resulting in the formation of seeds which grow into a new hybrid. The in vitro seed germination of hybrid seeds of Renanthera bella x Vanda helvola; Paraphalaenopsis denevei x Phalaenopsis bellina; and V. dearei x V. helvola were done by using half strength MS medium supplemented with 10% v/v of coconut water, 10% w/v of tomato juice, 10% w/v of potato homogenate and a control treatment. For 30% germination, R. bella hybrid seeds took 10 days after culture on half-MS supplemented with coconut water, P. denevei hybrid 14 days after culture on half-MS without additive, and V. dearei hybrid took 14 days after culture on half-MS supplemented with potato homogenate. All cultures from the entire studies were maintain under 24 hour light conditions at 24±2 Dc. The analysis of variance (ANOVA) confirmed that there is a significant effect of complex additives on the in vitro germination of all the three hybrids seeds at 20-60 days after culture.
| Item Type: | Academic Exercise |
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| Keyword: | Orchid, Hybrid, Genetic, Orchid plant, Renanthera bella x Vanda helvola, Paraphalaenopsis denevei x Phalaenopsis bellina, V. dearei x V. helvola |
| Subjects: | Q Science > QK Botany > QK1-989 Botany > QK474.8-495 Spermatophyta. Phanerogams |
| Department: | SCHOOL > School of Sustainable Agriculture |
| Depositing User: | ADMIN ADMIN |
| Date Deposited: | 22 Oct 2012 16:04 |
| Last Modified: | 13 Oct 2017 12:10 |
| URI: | https://eprints.ums.edu.my/id/eprint/5099 |
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