Joanna W. Doinsing (2005) Random Amplified Polymorphic DNA (RAPD) Fingerprinting Of Marine Harmful Algae, Genus Pseudo-Niizschia. Universiti Malaysia Sabah. (Unpublished)
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Abstract
In this study, the Random Amplified Polymorphic DNA (RAPD) technique was used to distinguish three algae species of the genus Pseudo-nitzschia; PNA, PNB and PNC. Genomic DNAs of the three species were first extracted by using modified method of phenol extraction and ethanol precipitation. Of the 16 random primers tested, only 14 amplified DNA segments (OPA-1, OPA-3-5, OPA-7-1O, OPA-12, OPA-14, OPA-16 and OPA-18-20.). From the selected 14 primers, OPA-16 and OPA-18 primers were used for genetic similarity and genetic distance analysis as the bands produced were clear, distinct and reproducible. Genetic similarity and genetic distance of the three species of Pseudo-nitzschia was then calculated by using the Nei and Li (1979) equation. The genetic similarity observed between PNA and PNB was 0.5941, followed by PNB and PNC with value 0.5012 and finally PNA and PNC with value 0.4143 while the genetic distance calculated between PNA and PNB was 0.4059, followed by PNB and PNC with value 0.4988 and finally PNA and PNC with value 0.5857. Cluster analysis of distance values was conducted to construct a dendrogram. From the analysis, it was found that the Pseudo-nitzschia species; PNA was very much closer to PNB compared to PNC.
| Item Type: | Academic Exercise |
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| Keyword: | Random Amplified Polymorphic DNA, algae, Pseudo-nitzschia, phenol extraction, ethanol precipitation |
| Subjects: | Q Science > QH Natural history > QH301-705.5 Biology (General) |
| Department: | SCHOOL > School of Science and Technology |
| Depositing User: | SITI AZIZAH BINTI IDRIS - |
| Date Deposited: | 21 Nov 2013 15:48 |
| Last Modified: | 17 Oct 2017 13:23 |
| URI: | https://eprints.ums.edu.my/id/eprint/7478 |
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