Mohd. Zuhair Zainal Abidin (2007) Percambahan renanthera bella secara in vitro dan pertumbuhan serta perkembangan protokom. University Malaysia Sabah. (Unpublished)
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Abstract
This study was carried out to evaluate the effect of different culture media on germination of and growth and development of protocorms. The culture medium choosen for this in vitro germination of R. bella was Experimental Ernst Robert medium (XER). The seed was obtained from hand pollinated R. bella pods. A Half strength and full strength of MS vitamins were used as an additive in the basal XER medium. XER medium without any additional vitamin was used as control medium. Proto corms formed from the germinated seed were recultured on other medium to observe the growth and development. The culture media used for this purpose were Murashigee & Skoog (MS) and Vacin & Went (VW). The different BAP at concentrations 0, 1.0 and 3.14 μM wereused to induced the development of protocorm which were 0 μM, 1.0 μM and 3.14 μM. Each different concentration of hormone was combined with another 4 concentrations of coconut water. Concentration of coconut water used were 0% (v/v), 10% (v/v), 15% (v/v) and 20 % (v/v). Solely MS medium and VW medium without any addition of hormone and coconut water were used as control treatment. XER medium with half strength of vitamin MS had the most striking effect on inducing germination of R. bella compared to others. For the protocorm growth and development, VW medium containing 3.14 μM BAP formed high percentage of shoot formation but 1.0 μM BAP also recorded high mean numbers of shoot in total treatment. The 20% (v/v) coconut water generally gave a significant effect on proliferation of protocorm in combination with 1.0 μM BAP.
| Item Type: | Academic Exercise |
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| Keyword: | germination, culture media, protocorm, Experimental Ernst Robert medium (XER), Murashigee & Skoog (MS), Vacin & Went (VW), proliferation, treatment |
| Subjects: | Q Science > QK Botany |
| Department: | SCHOOL > School of Science and Technology |
| Depositing User: | SITI AZIZAH BINTI IDRIS - |
| Date Deposited: | 15 May 2014 11:04 |
| Last Modified: | 13 Oct 2017 12:00 |
| URI: | https://eprints.ums.edu.my/id/eprint/9031 |
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