Thau, Wilson,Lym Yong and Siew, Hoo Ting and Wei, Lie Chin and Kenneth Francis Rodrigues and Ann Anton, Datin (2011) In vitro Micropropagation of Eucheuma Seaweeds. In: International Conference on Biotechnology and Food Science (ICBFS) 2011, 01-03 April 2011, Bali Island , Indonesia. (Submitted)
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Abstract
The cultivation of seaweeds contributes significantly to the national economy and provides employment to the economically backward communities of Sabah, Malaysia. Cultivation of seaweeds on a commercial scale requires a large number of propagules with desirable phenotypic traits which include high growth rates and resistance to diseases. Thus, seaweed tissue culture can be considered one of the best methods to provide a large amount of seedlings for cultivation. The parameters which have been determined to have a significant effect on the growth of in vitro propagated Echeuma seaweeds are the aseptic culture techniques, media ~position, light intensity, and aeration. Eucheuma seaweeds Were surface sterilized by Washing with optimized concentration of disinfectants and antibiotics. The appropriate medium was determined to be Provasoli's Enriched Seawater (PES) while optimum light intensity was in the range of 6,000 lux. Continuous aeration was important to provide enough carbon dioxide for carbon fixation. Hormone IAA:BAP was provided with concentration ratio of 5: 1 mg/I. Temperature was maintained at the range of 25 to 30°C while salinity of the seawater was constant at 32 ppt.
| Item Type: | Conference or Workshop Item (UNSPECIFIED) |
|---|---|
| Keyword: | Eucheuma, micropropagation, tissue culture |
| Subjects: | S Agriculture > SH Aquaculture. Fisheries. Angling |
| Depositing User: | MDM FAUZIAH MATSIN |
| Date Deposited: | 23 Jun 2014 13:21 |
| Last Modified: | 09 Nov 2017 16:01 |
| URI: | https://eprints.ums.edu.my/id/eprint/9239 |
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