Sequence analysis of 16S rRNA gene and 16S-23S rRNA gene intergenic spacer region for differentiation of probiotics Lactobacillus strains isolated from the gastrointestinal tract of chicken

Chin, Mei Lee and Chin, Chin Sieo and Wong, Clemente Michael Vui Ling and Noraini Abdullah and Yin, Wan Ho (2008) Sequence analysis of 16S rRNA gene and 16S-23S rRNA gene intergenic spacer region for differentiation of probiotics Lactobacillus strains isolated from the gastrointestinal tract of chicken. Annals of Microbiology, 58 (1). pp. 133-140. ISSN 1590-4261

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Abstract

Twelve probiotic Lactobacillus strains which were previously identified with classical biochemical tests were re-identified using molecular methods. Comparative sequence analyses of the 16S rRNA gene and 16S-23S rRNA gene intergenic spacer region (ISR) were applied. Results of the study showed that mis-identification at species level occurred at high rate when classical biochemical tests were used. Nine of the strains showed discrepancy in their identity. These nine strains which were previously identified through biochemical tests as L. brevis C1, L. brevis C10, L. fermentum C16, L. brevis C17, L. crispatus I12, L. acidophilus I16, L. fermentum I24, L. fermentum I25 and L. acidophilus I26 were re-identified as L. reuteri C1, L. reuteri C10, L. reuteri C16, L. panis C17, L. brevis I12, L. gallinarum I16, L. salivarius I24, L. brevis I25 and L. gallinarum I26, respectively, using 16S rRNA gene and 16S-23S rRNA gene ISR analysis. Lactobacillus strains I16 and I26 initially could not be classified into a single taxon by 16S rRNA gene sequencing but the identities of these two strains were eventually resolved by 16S-23S rRNA gene ISR sequence analysis as L. gallinarum. Sequence analysis of 16S rRNA gene in complementary with 16S-23S rRNA gene ISR could be potentially useful for rapid and reliable identification of bacteria.

Item Type:	Article
Keyword:	16s rRNA gene, 16S-23S rRNA gene intergenic spacer region (ISR), Lactobacillus spp, Phylogenetic analysis
Subjects:	Q Science > QR Microbiology > QR1-502 Microbiology > QR75-99.5 Bacteria S Agriculture > SF Animal culture > SF1-1100 Animal culture > SF481-507 Poultry. Eggs
Department:	INSTITUTE > Biotechnology Research Institute (BRI)
Depositing User:	ADMIN ADMIN
Date Deposited:	05 Apr 2011 15:39
Last Modified:	30 Aug 2021 22:26
URI:	https://eprints.ums.edu.my/id/eprint/2691

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