Siti Aishah Mohd Ali and Jalifah Latip (2021) Rapid investigation of the metabolite content in Hibiscus sabdariffa var. UKMR-2 cultivated under the influence of elevated co2 using tri-step ft-ir spectroscopy. Jurnal Teknologi, 83 (1). pp. 75-83. ISSN 2180–3722
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Rapid investigation of the metabolite content in Hibiscus sabdariffa var. UKMR-2 cultivated under the influence of elevated co2 using tri-step ft-ir spectroscopy ABSTRACT.pdf Download (113kB) |
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Rapid investigation of the metabolite content in Hibiscus sabdariffa var. UKMR-2 cultivated under the influence of elevated co2 using tri-step ft-ir spectroscopy FULL TEXT.pdf Restricted to Registered users only Download (624kB) | Request a copy |
Abstract
Rapid methods based on untargeted analysis technique such as Fourier Transform Infrared (FT-IR) spectroscopy can provide much faster and easier solution for food authentication. However, studies on the metabolite content in UKMR-2 calyces using FT-IR spectroscopy has not been reported yet in any previous studies. Thus, the present study was performed to analyze the differences in metabolite content in UKMR-2 calyces under the influences of different [CO2] treatment by applying tri-step infrared based fingerprinting. The UKMR-2 plant cultivation was exposed to ambient [CO2] (400 μmol/mol) and elevated [CO2] (800 μmol/mol) treatment. The UKMR-2 calyx extracts were analysed by conventional infrared (1D-IR), second derivative infrared (SD-IR) and two-dimensional correlation infrared (2D-IR) spectroscopy. The 1D-IR spectrum results revealed a similar absorption spectrum in the range of 1900 - 650 cm-1, which suggest similar major metabolites content present in both extracts. For SD-IR spectrum, both treatments clearly showed have more peaks with different shape, position and intensity in the range of 1650 - 1450 cm-1 and 1200 - 950 cm-1, which is likely to have different flavonoid and carbohydrate content in UKMR-2 calyces. The 2D-IR synchronous correlation spectrum in the range of 1000 – 650 cm-1 clearly distinguished the metabolite content in the UKMR-2 calyx extract from different [CO2] treatment. Therefore, this tri-step infrared based fingerprinting has the potential as one of the effective methods to discriminate extract samples with similar infrared fingerprint features and indicate that the metabolite content in UKMR-2 calyces were influenced by different [CO2] treatments.
| Item Type: | Article |
|---|---|
| Keyword: | H. sabdariffa var. UKMR-2 , Elevated [CO2] , Infrared Based Fingerprinting , Second Derivative Infrared , 2D-IR Synchronous Correlation |
| Subjects: | S Agriculture > SB Plant culture T Technology > TX Home economics > TX1-1110 Home economics > TX341-641 Nutrition. Foods and food supply |
| Department: | FACULTY > Faculty of Social Sciences and Humanities |
| Depositing User: | SAFRUDIN BIN DARUN - |
| Date Deposited: | 14 Jun 2021 15:19 |
| Last Modified: | 14 Jun 2021 15:19 |
| URI: | https://eprints.ums.edu.my/id/eprint/27245 |
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