Isolation and characterization of lactic acid bacteria from Sabah (North Borneo) stingless bees for probiotic and food applications

Goh, Lucky Poh Wah and Arnold Marshall Molujin and Kaliswaran Muthu and Rahmath Abdulla and Mohd Khalizan Sabullah and Ainol Azifa Mohd Faik and Jualang Azlan Gansau and Roslina Jawan (2021) Isolation and characterization of lactic acid bacteria from Sabah (North Borneo) stingless bees for probiotic and food applications. International Journal of Food Properties, 24. pp. 564-578. ISSN 1094-2912 (P-ISSN) , 1532-2386 (E-ISSN)

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URL: https://www.tandfonline.com/doi/full/10.1080/10942...

Abstract

Stingless bees harbor lactic acid bacteria (LAB) which possesses multiple beneficial properties. However, there is no report on LAB in stingless bee from Sabah and their products. This study aimed to isolate LAB from several stingless bee species and their products as well as to perform characterization and safety assessments. A total of 104 strains were isolated and seven potential antimicrobial LAB isolates were identified from stingless bee and their products. Characterization, identification, and assessments were performed on seven (A2b, B3b, P1b, H4b, A6, B5, and B10) LAB that exert potential antimicrobial activity against Listeria monocytogenes ATCC 7644. The A6 isolate was closely related to the Lactobacillus pentosus species whereas isolates B5 and B10 were closely related to Weissella paramesenteroides species. Finally, isolates P1b, H4b, B3b, and A2b were closely related to the Enterococcus sp. These seven LAB isolates were able to survive in stimulated gastrointestinal tract conditions (acidic, salt, bile salt, and temperature). The carbon fermentation, proteolytic activities, acidification, milk coagulation, and wide antibiotic susceptibility testing of seven LAB isolates revealed their potential used as a probiotic and fermentation purposes. Therefore, more studies are warranted to investigate the potential of these LAB isolates towards applications in probiotic and fermentation.

Item Type:	Article
Keyword:	Lactic acid bacteria , Stingless bee , Food and probiotic , Antimicrobial , Bacteria , Foaming capacity , Functional properties , Milk
Subjects:	T Technology > TX Home economics > TX1-1110 Home economics > TX341-641 Nutrition. Foods and food supply
Department:	FACULTY > Faculty of Science and Natural Resources
Depositing User:	SAFRUDIN BIN DARUN -
Date Deposited:	28 Jul 2021 10:31
Last Modified:	28 Jul 2021 10:31
URI:	https://eprints.ums.edu.my/id/eprint/30109

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