Jaeyres Jani and Zainal Arifin Mustapha and Norfazirah Jamal and Cheronie Shely Stanis and Chin Kai Ling and Richard Avoi and Naing Oo Tha and Valentine Gantul and Daisuke Mori and Kamruddin Ahmed (2019) Whole genome sequencing data and analysis of a rifampicin-resistant mycobacterium tuberculosis strain sbh162 from Sabah, Malaysia.
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Whole genome sequencing data and analysis of a rifampicin-resistant Mycobacterium tuberculosis strain SBH162 from Sabah, Malaysia-Abstract.pdf Download (71kB) |
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Whole genome sequencing data and analysis of a rifampicin-resistant Mycobacterium tuberculosis strain SBH162 from Sabah, Malaysia.pdf Restricted to Registered users only Download (645kB) | Request a copy |
Abstract
A Mycobacterium tuberculosis strain SBH162 was isolated from a 49-year-old male with pulmonary tuberculosis. GeneXpert MDR/ RIF identified the strain as rifampicin-resistant M. tuberculosis. The whole genome sequencing was performed using Illumina HiSeq 4000 system to further investigate and verify the mutation sites of the strain through genetic analyses namely variant calling using bioinformatics tools. The de novo assembly of genome generated 100 contigs with N50 of 156,381bp. The whole genome size was 4,343,911 bp with G þ C content of 65.58% and consisted of 4,306 predicted genes. The mutation site, S450L, for rifampicin resistance was detected in the rpoB gene. Based on the phylogenetic analysis using the Maximum Likelihood method, the strain was identified as belonging to the Europe America Africa lineage (Lineage 4). The genome dataset has been deposited at DDBJ/ENA/GenBank under the accession number SMOE00000000.
| Item Type: | Article |
|---|---|
| Additional Information: | The data will shed light on the molecular biology of a Mycobacterium tuberculosis strain, which will be beneficial to researchers working on tuberculosis. The data will give insight into drug resistance in M. tuberculosis, which will benefit clinicians and patients. The data will help to understand the relation between M. tuberculosis strains from Sabah and other areas, which will contribute to policy making for the control of tuberculosis. |
| Keyword: | M. tuberculosis , Whole genome sequencing , Next generation sequencing , Rifampicin resistant , Sabah , Malaysia |
| Subjects: | G Geography. Anthropology. Recreation > GE Environmental Sciences > GE1-350 Environmental sciences Q Science > QR Microbiology > QR1-502 Microbiology |
| Department: | FACULTY > Faculty of Medicine and Health Sciences |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 20 Sep 2021 08:31 |
| Last Modified: | 19 May 2023 10:58 |
| URI: | https://eprints.ums.edu.my/id/eprint/30674 |
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