Kenneth Francis Rodrigues and Ahmad Zaidi Tani and Syarul Nataqain Baharum (2016) Recruitment of grouper broodstock on the basis of single locus DNA markers. International Journal of Fisheries and Aquatic Studies, 4. pp. 201-208. ISSN 2394-0506 ( P-ISSN) , 2347-5129 (E-ISSN) (In Press)
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Recruitment of grouper broodstock on the basis of single locus DNA markers-ABSTRACT.pdf Download (60kB) |
Abstract
Scientific breeding programs are founded on the screening and recruitment of genetically diverse broodstock, with the ultimate aim of developing heterogeneous breeding populations that host a collection of desirable traits. Single locus DNA markers can be applied to facilitate the process of selection as they are species specific, reliable, reproducible and easy to use. This study set forth to develop a library of single locus DNA markers for two commercially cultured species of groupers, Epinephelus fuscoguttatus and Epinephelus corallicola. DNA was isolated from one representative specimen of each species and utilized to construct shotgun genomic libraries. DNA sequences derived from the library were selected for the development of 42 and 41 single locus DNA markers for Epinephelus fuscoguttatus and Epinephelus corallicola respectively. The markers were then tested against randomly selected specimens obtained from the wild. Genotyping results revealed that the species specific primers demonstrated the ability to distinguish between individuals from the same species into distinct operational taxonomic units (OTUs) on the basis of their differential DNA profiles, thus establishing a basis for selection based on genetic heterogeneity. The findings of this study present a strong case for the application of single locus DNA markers as molecular tools for the selection of broodstock on the basis of genotyping.
| Item Type: | Article |
|---|---|
| Keyword: | Grouper , Epinephelus fuscoguttatus , Epinephelus corallicola , Single locus DNA markers |
| Subjects: | H Social Sciences > HD Industries. Land use. Labor > HD28-9999 Industries. Land use. Labor > HD9000-9999 Special industries and trades > HD9000-9495 Agricultural industries Q Science > QL Zoology > QL1-991 Zoology > QL605-739.8 Chordates. Vertebrates > QL614-639.8 Fishes |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 26 Oct 2021 16:00 |
| Last Modified: | 26 Oct 2021 16:00 |
| URI: | https://eprints.ums.edu.my/id/eprint/30733 |
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