Siti Nurnatasya Raihana Mohd Yusrie and Roslina Jawan (2022) Influence of protective agents and storage conditions on cell viability of probiotic lactic acid bacteria, lactobacillus mendensis ca4. In: International conference on food science and nutrition 2022, 24 - 25 August 2022, Virtual Conference.
![[img]](https://eprints.ums.edu.my/style/images/fileicons/text.png) |
Text
ABSTRACT.pdf Download (58kB) |
|
Text
FULLTEXT.pdf Restricted to Registered users only Download (231kB) | Request a copy |
Abstract
This study aims to evaluate the influence of protective agents on cell viability of Lactobacillus mindensis CA4 after freeze-drying process and during the storage at various temperatures. The protective agents used were skim milk, beef extract, soybean, and yeast extract at 10 % (w/v), respectively. Cell viability and survival rate were determined after the freeze-drying processes and during the storage at various temperatures (-20, 4 and 25 ˚C) up to 15 days. The residual moisture content and morphological of the freeze-dried samples were also analysed. Results show that after the freeze-drying, the highest cell survivability rate was recorded in skim milk (77.40%) followed by yeast extract (47.25%), and beef extract (42.86%) with cell viability at 2.07 x 106 , 9.83 x 105 , and 5.67 x 105 CFU/mL, respectively. All freeze-dried cells exhibited better survivability at storage condition -20 ˚C as compared to 4 and 25 ˚C. During storage, the cell viability in all protective agents were lower than 106 CFU/mL except for skim milk (1.12 x 106 CFU/mL) on the 10th day of storage at -20 ˚C. The cell viability of freeze-dried cells in skim milk was reduced to 6.33 x 105 CFU/mL at day 15th of storage at - 20 ˚C. Skim milk recorded the lowest moisture content (0.11%) with the highest survivability rate (77.40%), showing an inversed relationship. In future the findings of this study could be used to develop a potential formulation of protective agent for commercialization of probiotic and bioprotective cultures.tra
| Item Type: | Conference or Workshop Item (Paper) |
|---|---|
| Keyword: | Probiotic , Lactic acid bacteria , Freeze-drying , Cell viability , Protective agents |
| Subjects: | Q Science > Q Science (General) > Q1-390 Science (General) Q Science > QR Microbiology > QR1-502 Microbiology > QR1-74.5 General |
| Department: | FACULTY > Faculty of Science and Natural Resources |
| Depositing User: | ABDULLAH BIN SABUDIN - |
| Date Deposited: | 13 Feb 2023 15:04 |
| Last Modified: | 22 May 2023 11:36 |
| URI: | https://eprints.ums.edu.my/id/eprint/35079 |
Actions (login required)
 |
View Item |