Lia Natasha Amit (2018) Detection of community-acquired methicillin-resistant staphylococcus aureus (CA-MRSA) transmission in the local community using molecular typing techniques. Masters thesis, Universiti Malaysia Sabah.
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Abstract
Since the 1990s, Community-acquired Methicillin-Resistant Staphylococcus aureus (CA-MRSA) has caused infections in population without healthcare-associated risk factors. Unlike nosocomial MRSA, CA-MRSA harbors pv/gene and SCCmedV subtype, V, or untypable SCCmec. CA-MRSA is usually associated with suppurative infections among children. Children are more predisposed to be CA-MRSA carriers and transmit CA-MRSA to family members. This study aimed to elucidate CA-MRSA transmission in Sabah local community by molecular characterization of MRSA abscess isolates from pediatric patients in Hospital Likas and MRSA nasal isolates from participating family members within a one-year period. Thirty-seven MRSA abscess isolates of index patients which were identified from the cefoxitin antibiotic susceptibility tests were collected from Pathology Laboratory. Nineteen nasal swab samples were collected from family members and processed by the culture on blood agar, Gram staining, catalase test, coagulase test, and DNAse test for 5. aureus identification. PCR of mecA gene were conducted on 37 MRSA abscess isolates and 14 S. aureus nasal isolates. PCR of pv/gene and PCR for SCCmectyping were conducted on MRSA isolates. PVL positive CA-MRSA isolates of index patients and family members which did not harbor similar SCCmec elements were tested for spa typing. Patients' age, ethnicity, and site of infection were recorded into patient information sheets. Research survey were given to participating family members. This study validated the methicillin resistance status of 37 MRSA abscess isolates and 14 nasal 5. aureus based on the presence of mecA gene. This study supported previous studies where CA-MRSA predominated in abscess infections of pediatric patients. From 37 MRSA abscess isolates, 33 isolates were confirmed as CA-MRSA based on the presence of pv/gene and SCCmedVa (n=12), IVc (n=l), V (n=l), or untypable SCCmec(n=19). From 37 index cases, only CA-MRSA isolates from 2 cases showed similarities of spa genes. CA-MRSA abscess isolates, Al 167 (S2) and AS413 (S7), shared similar spa types with CA-MRSA isolates from family members, F1167 (S2.1) and F5413 (S7.1), respectively. From 37 CA-MRSA index cases, 18 patients had abscesses on scalp suggesting that infant locomotion development, skin-to-skin contact, and contaminated household surfaces leads to CA-MRSA transmission. This study has extended the understanding of the CA-MRSA transmission. The data herein could be important in developing antidotes for CA-MRSA infections in Malaysia.
| Item Type: | Thesis (Masters) |
|---|---|
| Keyword: | Staphylococcus aureus, Population, Local community |
| Subjects: | Q Science > QR Microbiology > QR1-502 Microbiology |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 23 Apr 2024 14:50 |
| Last Modified: | 23 Apr 2024 14:50 |
| URI: | https://eprints.ums.edu.my/id/eprint/38510 |
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