Enzymatic hydrolysis of protein hydrolysate from Pangasius sp. by-product using bromelain

Rahmi Nurdiani and Muhamad Firdaus and Asep Awaludin Prihanto and Abdul Aziz Jaziri and Muhammad Rayhansyah Jati and Taufiq Rizki Abdurrahman and Syaravina Ifilah and Elfriede Rositta Debataraja and Nurul Huda (2024) Enzymatic hydrolysis of protein hydrolysate from Pangasius sp. by-product using bromelain. Current Research in Nutrition and Food Science, 12 (1). pp. 1-12. ISSN 2347-467X

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Abstract

Fish protein hydrolysate (FPH) is a product resulting from the degradation of fish protein into simple peptides and amino acids through hydrolysis. This study aims to optimise the enzymatic hydrolysis conditions of Pangasius sp. by-products to produce high-quality fish protein hydrolysate. Bromelain enzyme was used as the catalyst for hydrolysis. The degree of hydrolysis (DH), pH and antioxidant activity of FPH were used as response parameters. The optimisation was done using response surface methodology (RSM) by applying two factors (enzyme concentration and incubation time) with a 3-level Central Composite Design (CCD) model. The result showed that the bromelain concentration and incubation time gave significantly different effects (p<0.05) on the response parameters of Pangasius protein hydrolysate. Hydrolysis of Pangasius protein with 0.04% bromelain enzyme and incubation time of 2.8 hours resulted in DH, pH and DPPH antioxidant activity of 35.88%, 7.07 and 29.86%, respectively. The response value of Pangasius protein hydrolysate was within the range of the predicted value of hydrolysate. Therefore, the optimum conditions suggested by RSM can be used in the future production of Pangasius FPH. In addition, amino acid profiles of Pangasius protein hydrolysate showed high concentrations of Glycine, L-glutamic acid and L-aspartic Acid.

Item Type:	Article
Keyword:	Bromelain; Fish Protein Hydrolysate; Optimisation; Pangasius By-Product.
Subjects:	Q Science > QP Physiology > QP1-(981) Physiology > QP1-345 General Including influence of the environment T Technology > TX Home economics > TX1-1110 Home economics > TX341-641 Nutrition. Foods and food supply
Department:	FACULTY > Faculty of Sustainable Agriculture
Depositing User:	ABDULLAH BIN SABUDIN -
Date Deposited:	05 Aug 2024 11:16
Last Modified:	05 Aug 2024 11:16
URI:	https://eprints.ums.edu.my/id/eprint/39452

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