Ignatia Justine (2023) Co-cultivation of yeast and microalgae on pome for simultaneous cod reduction and lipid production. Masters thesis, Universiti Malaysia Sabah.
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Abstract
Palm oil mill effluent (POME) contains high chemical oxygen demand (COD) which needs to be treated before discharge to waterbodies. Oleaginous yeast and microalgae have been known capable to grow and utilize nutrients present in POME resulting in COD reduction besides the benefits of microbial oils production as an alternative source for biodiesel. Thus, this study will focus on the cultivation of yeast (Rhodotorula toruloides ) and microalgae (Ankistrodesmus fusiformis ) on POME as a cheap culture medium to stimulate cell growth. It is hypothesized that utilizing POME as cheap substrate medium will effectively aid the cell growth of R. toruloides and A. fusiformis . Moreover, the cocultivation of R. toruloides and A. fusiformis in POME will improve the COD reduction and lipid production as biodiesel feedstock via synergistic activity, as compared to a single culture. The optimum conditions [POME concentration (%), growth duration (days), and yeast to algae ratio] were optimized using Response Surface Methodology (RSM) through Design Expert Software (DOE) and analysed based on COD concentration (mg/L) lipid productivity (mg/ml/day), and biomass yield (mg/ml). Based on this study, co-cultivation in POME outperformed single culture with highest COD reduction efficiency and lipid production by 87.52% (4,235 mg/L) and 15.39 mg/ml/day respectively, whereas the COD reduction and lipid production by R. toruloides and A. fusiformis were 43%, 47%, and 4.51%, 1.67% correspondingly. Based on the analysis, all models showed significant result with p-value of p<0.05. In addition, the optimum conditions for COD removal and lipid production were 59.88% of POME concentration, 0.36 (9:25) yeast to microalgae ratio, and 18 days of growth duration. Thus, these findings show the utilization of POME as a cheap culture medium has enhanced the COD reduction and lipid production by co-cultivation as compared to single culture. In the future, the study in-depth based on the optimized condition of this co-culture would contribute to greater COD reduction and lipid production by applying more alternative method such as application of a catalyst.
| Item Type: | Thesis (Masters) |
|---|---|
| Keyword: | Palm oil mill effluent, Yeast, Microalgae, Microbial oils production, Biodiesel. |
| Subjects: | T Technology > TP Chemical technology > TP1-1185 Chemical technology |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 02 Sep 2024 15:39 |
| Last Modified: | 02 Sep 2024 15:39 |
| URI: | https://eprints.ums.edu.my/id/eprint/40775 |
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