Effects of solvent system and drying on the total phenolic content and antioxidant activities of leaf extract of Pereskia bleo.

Hwei Chyi Lui and Ai Ling Ho and Jau-Shya Lee and Pei-Teng Chan and Chee Kiong Siew (2024) Effects of solvent system and drying on the total phenolic content and antioxidant activities of leaf extract of Pereskia bleo. Transactions on Science and Technology, 11 (4-2). pp. 1-6. ISSN 2289-8786

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Abstract

Pereskia bleo (P. bleo), a plant traditionally consumed in parts of China, Malaysia, and Panama, is believed to have a variety of medicinal properties, including anti-cancer, anti-tumour, anti-rheumatic, anti-ulcer, and anti-inflammatory effects. The total phenolic content (TPC) and antioxidant activities of the leaves of P. bleo were determined using three extraction solvents of different polarities: water, methanol and dichloromethane. The effects of drying in a drying oven at 40˚C on the TPC and antioxidant activities were also determined. TPC was measured using the Folin-Ciocalteu reagent, and antioxidant activities were determined using ferric reducing antioxidant power (FRAP) assay and beta-carotene bleaching (BCB) assay. While the aqueous extract exhibited the highest TPC (p<0.05) (34.20 mg GAE/g extract for fresh leaves and 71.24 mg GAE/g extract for dried leaves), the methanolic extract showed the strongest antioxidant activities (p<0.05) for both the FRAP assay (332.17mmol Fe (II)/g extract for fresh leaves and 270.59 mmol Fe (II)/g extract for dried leaves) and the BCB assay (69.86% for fresh leaves and 60.50% for dried leaves). Drying resulted in a lower TPC (p<0.05) for the methanol and dichloromethane extractions (-5.39% and -15.67%, respectively) but an increased TPC (p<0.05) for the water extraction (+52.00%). The FRAP and BCB activities decreased (p<0.05) after drying, with reductions ranging from -12.82% to -34.53% and -13.31% to -65.52%, respectively.

Item Type:	Article
Keyword:	Pereskia bleo; Total phenolic content; Antioxidant activity; Ferric reducing antioxidant power; Beta-carotene bleaching
Subjects:	Q Science > QK Botany > QK1-989 Botany T Technology > TA Engineering (General). Civil engineering (General) > TA1-2040 Engineering (General). Civil engineering (General) > TA401-492 Materials of engineering and construction. Mechanics of materials
Department:	FACULTY > Faculty of Food Science and Nutrition
Depositing User:	ABDULLAH BIN SABUDIN -
Date Deposited:	16 May 2025 12:33
Last Modified:	16 May 2025 12:33
URI:	https://eprints.ums.edu.my/id/eprint/43735

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