Isolation, identification and characterisation of bacteria from deception island, Antarctic

Jessica Teo Siaw Fui (2012) Isolation, identification and characterisation of bacteria from deception island, Antarctic. Masters thesis, Universiti Malaysia Sabah.

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Abstract

Deception Island, an active stratovolcano is one of the South Shetland Islands in the Antarctic. It provides excellent conditions for thermophilic bacteria to grow because of high ground temperatures in specific areas, where the temperatures are above the mesophilic range. The aims of this study were to isolate some thermophiles from two locations in the Deception Island, and to determine their enzyme profiles. Eight strains of bacteria Bl, 82, 83, 84, BS, 86, 87 and 88 were isolated at a growth temperature of 40° C. The eight strains were Gram-positive bacilli and harboured endospores. These bacteria were identified based on their 16S rDNA sequences. Three of them were duplicates of the other strains and therefore only five were used for the subsequent analyses. The eight bacterial strains Bl, B2 (and B8), B3, B4 (and 85), and 86 (and 87) had the highest percentage similarities to Paenibacl'llus barengoltzii (96%), Aneurinibaci/lus danicus (92%), Aneurinibacil/us migulanus (91 %) Paenibaci/lus timonensis (96%) and Bacillus weihenstephanensis (99%) respectively. Those with less than 97% similarity to known species are possibly novel species. The growth rates of the five bacterial strains were highest at 37°C when compared to 40° C, 50° C and 60° C. Strain Bl was found to produce amylase, xylanase, and cellulase. Strain B2 was able to produce amylase and cellulase. Strain 83 was able to produce amylase, xylanase and cellulase while strain B4 was found to produce amylase, xylanase and cellulase. The enzyme produced by these bacteria may be useful for the industries.

Item Type:	Thesis (Masters)
Keyword:	Thermophilic bacteria, Deception Island, Antarctica microbiology, Bacillus species, 16S rDNA sequencing
Subjects:	Q Science > QR Microbiology > QR1-502 Microbiology > QR1-74.5 General
Department:	INSTITUTE > Biotechnology Research Institute (BRI)
Depositing User:	DG MASNIAH AHMAD -
Date Deposited:	17 Jun 2025 11:25
Last Modified:	17 Jun 2025 11:25
URI:	https://eprints.ums.edu.my/id/eprint/44140

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