Nor Amirah Shamsudin (2018) Bioactive properties and phytochemical analysis of Rubus fraxinifolius poir (Rogimot). Masters thesis, Universiti Malaysia Sabah.
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Abstract
Rubus fraxinifolius, locally known as Rogimot, is an underutilized edible fruit that can be found distributed from low tropic to subtropics region. The aims of the present study were to determine the antioxidant activities, phytochemicals content, anticholinesterase and antibacterial properties in different parts (i.e., fruit, stem, and leaves) of the plants. Antioxidants were analysed using Ferric Reducing Antioxidant Power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay. All samples were freezedried and extracted using five different solvents (dH20, absolute ethanol, 80% (v/v) ethanol, absolute methanol, and 80% (v/v) methanol). An antioxidant test showed that 80 (%) methanol crude extract display a higher antioxidant value compares to the other extract. The extract of 80% methanol is then subjected to phytochemicals analysis, where the result of total phenolic and total flavonoid content is found higher in the leaves part with the values of 56.32±0.05 mg GAE/g and 31.36±1.05 mg CE/g, respectively. Meanwhile, fruit part showed the highest total anthocyanin and total carotenoid content with values of 22.27±1.28x10-14mg C-3-GE/ g and 10.02±0.22 mg BC/g, respectively. For AChE inhibition activity, enzyme inhibition method is used with galanthamine as a positive control. The result of 80% (v/v) methanol extract of fruit, stem, and leaves showed a dependent inhibition activity against the AChE enzyme, where leaves part showed the highest ACh inhibition activity (63.48±0.48%) when tested at 250 µg/ml, followed by stem and fruit part. The leaves part is chosen for phytochemical analysis and were extracted with liquid-liquid extraction before undergoes fractionation by column chromatography. A total of 10 fractions were screened for their antioxidant, anticholinesterase, and antimicrobial activity. Nevertheless, fraction 5 and fraction 6 showed the highest antioxidant, AChE inhibition, and antibacterial activity. Therefore, both fractions are subjected to GC/MS analysis. The result on GC/MS analysis of both fractions revealed a total of 5 major compounds and among the major compounds that had been identified from both fraction is a phenol group compound, i.e phenol, phenol, 2,4-bis(l,1-dimethylethyl)- and a fatty acid group, i.e hexadecanoic acid, which is widely known for their bioactivity. As a conclusion, R. fraximfolius leaves potentially provide protection against oxidation, antimicrobial, and having a potential as an acetylcholinesterase inhibitor.
| Item Type: | Thesis (Masters) |
|---|---|
| Keyword: | Rubus fraxinifolius, Rogimot, Underutilized fruit, Antioxidant activity, Phytochemicals |
| Subjects: | Q Science > QK Botany > QK1-989 Botany > QK474.8-495 Spermatophyta. Phanerogams |
| Department: | FACULTY > Faculty of Food Science and Nutrition |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 24 Jun 2025 15:07 |
| Last Modified: | 24 Jun 2025 15:07 |
| URI: | https://eprints.ums.edu.my/id/eprint/44249 |
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