Lai, Jason Sian Kang (2017) Response of marble goby (Oxyeleotris marmorata) to taste-stimulants for the development of weaning diet. Masters thesis, Universiti Malaysia Sabah.
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Abstract
Marble goby, 0xyeleotris marmorata is a high commercial value freshwater fish in Southeast Asia. In aquaculture, this fish generally rejects pelleted feed. This problem can be solved by dietary feeding stimulant (FS) or feed enhancer (FE) supplementation. However, there Is still no information on the suitable FS and FE for this fish. Therefore, the present study aimed to determine the potential FS and FE, and evaluate the efficiency of selected potential FS to improve pellet-weaning process of juvenile 0. marmorata. Prior to the behavioral assays, the fish were trained to accept agar gel pellet. Behavioral assays were conducted to determine the taste preference of 0. marmorata for 19 amino acids, 13 organic acids 3 nucleotides and nucleoside, 5 sugars and 2 classical taste substances at 0.1 M.' In addition, the optimal value of the taste substances identified with the highest total ingestion ratio (TIR) was determined. The role of betaine as FS and FE was also investigated. All taste substances were tested once to each of the so fish (hatchery-reared; Total length=6.4 to 8.8 cm) using agar gel pellet. Among the amino acids tested, aspartic acid attained highest TIR (0.94) with optimal level at 0.025 M. Among the nucleoside and nucleotides, inosine was the more potential FS as it attained perfect TIR (1) with optimal value of 0.001 M. All organic acids attained very high TIR (0.96 to 1) at 0.1 M but only citric acid, formic acid, fumaric acid, tartaric acid, acetic acid, maleic asid and malic acid remained with high TIR (0.86 to 1) at the lower concentration (0.01 M). All sugars, classical taste substances were totally rejected by the fish. Betaine was also determined to function neither as a FS or FE. In the next study, nine selected taste substances (aspartic acid, inosine, citric acid, formic acid, fumaric acid, oxalic acid, tartaric acid maleic acid and malic acid) were individually incorporated into the fish meal-based (FM) diet at 0.1 M, and their efficiency to improve the 19 day-weaning process were evaluated. The FM diet which contained no FS was prepared as the control. Each diet was fed to 20 replicates of juvenile 0. marmorata (hatchery-reared but naive-to-pellet; TL=4.9±0.4 cm). Each. fish was fed with 5 pieces of the pellet (size =2 mm x 2 mm X 2 mm) at 4 pm daily and the number of pellet eaten by each fish was recorded after 4 hours (8 pm). Among all treatments (were labeled using alphabets as data has been filed for trade secret), 'A" diet showed the highest daily mean ingestion ratios (DMIR) from day 5 to 19, with significant higher (P<0.05) IR than the other treatments on both day 9 and 19, indicating that the length of weaning period reduced when the fish was weaned to "A" diet. In addition, this treatment also attained highest percentage of successfully weaned fish which was 60%, and was significant (P<0.05) compared to other dietary treatment except "B" diet. Another similar weaning experiment was conducted to determine the optimal supplementation level of "A" using 0.01, 0.05, 0.2 M, and the results were compared to those of 0.1 M "A' from the previous weaning experiment. However, the DMIR of all diets showed similar trends to DMIR of control diet of previous weaning experiment, suggesting that 0.1 M could be the optimal level of "A" to function best as a FS. In conclusion, "A" substance with 0.1 M was detected as the best dietary FS and it can be used to improve the weaning process of juvenile o. marmorata.
| Item Type: | Thesis (Masters) |
|---|---|
| Keyword: | Oxyeleotris marmorata, Marble goby, Pellet weaning, Feeding stimulant |
| Subjects: | S Agriculture > SH Aquaculture. Fisheries. Angling > SH1-691 Aquaculture. Fisheries. Angling > SH20.3-191 Aquaculture > SH151-179 Fish culture |
| Department: | INSTITUTE > Borneo Marine Research Institute |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 03 Jul 2025 10:19 |
| Last Modified: | 03 Jul 2025 10:19 |
| URI: | https://eprints.ums.edu.my/id/eprint/44274 |
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