An overview of crosslinked enzyme aggregates: Concept of development and trends of applications

Oumaima Maroua Bouguerra and Roswanira Abdul Wahab and Fahrul Huyop and Abdo Mohammed Al‑Fakih and Wan Muhd Asyraf Wan Mahmood and Naji Arafat Mahat and Mohd Khalizan Sabullah (2024) An overview of crosslinked enzyme aggregates: Concept of development and trends of applications. Applied Biochemistry and Biotechnology, 196. pp. 5711-5739. ISSN 0273-2289

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Abstract

Enzymes are commonly used as biocatalysts for various biological and chemical processes in industrial applications. However, their limited operational stability, catalytic efficiency, poor reusability, and high-cost hamper further industrial usage. Thus, crosslinked enzyme aggre- gates (CLEAs) are developed as a better enzyme immobilization tool to extend the enzymes’ operational stability. This immobilization method is appealing because it is simpler due to the absence of ballast and permits the collective use of crude enzyme cocktails. CLEAs, so far, have been successfully developed using a variety of enzymes, viz., hydrolases, proteases, amidases, lipases, esterases, and oxidoreductase. Recent years have seen the emergence of novel strategies for preparing better CLEAs, which include the combi- and multi-CLEAs, magnetics CLEAs, and porous CLEAs for various industrial applications, viz., laundry deter- gents, organic synthesis, food industries, pharmaceutical applications, oils, and biodiesel pro- duction. To better understand the different strategies for CLEAs’ development, this review explores these strategies and highlights the relevant concerns in designing innovative CLEAs. This article also details the challenges faced during CLEAs preparation and solutions for overcoming them. Finally, the trending strategies to improve the preparation of CLEAs alongside their industrial application trends are also discussed.

Item Type:	Article
Keyword:	Enzymes, Immobilization, Crosslinked Enzyme Aggregates (CLEAs), Advantages, Disadvantages, Optimization of CLEAs
Subjects:	Q Science > QD Chemistry > QD1-999 Chemistry > QD241-441 Organic chemistry > QD415-436 Biochemistry
Department:	FACULTY > Faculty of Science and Natural Resources
Depositing User:	JUNAINE JASNI -
Date Deposited:	29 Jul 2025 10:11
Last Modified:	29 Jul 2025 10:11
URI:	https://eprints.ums.edu.my/id/eprint/44634

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