Tan, Pey Jiuan (2005) Antimicrobial potential of Lepiota sp. extract. Universiti Malaysia Sabah. (Unpublished)
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Abstract
The antimicrobial potential of Lepiota sp. extract in inhibiting the growth of pathogenic microbes isolated from human disease has been studied. Staphylococcus aureus, Escherichia coli and Candida albicans are the three microorganisms that were selected for this study. The objective of this study is to test for the antimicrobial activities of Lepiota sp. against Staphylococcus aureus, Escherichia coli and Candida albicans. This study also aimed to compare the factors that affect the effectiveness of the extract in antimicrobial activities. The factors which were considered in study are the concentrations of the extract, temperature of storage, period of storage and the mix time between the extract and the bacteria suspension. The value of Colony Forming Unit (CFU) was determined in the antimicrobial test. The results showed that Lepiota sp possessed antimicrobial potential in inhibiting the growth of S. aureus, E. coli and C. albicans. The extract was effective in decreasing the value of CFU of the microorganisms. The extract concentration of 350 mg/ml was proved to be the most effective in antimicrobial activities. The storage temperature and period of storage of the extract gave different effect to different microorganisms. In the antifungal test, Lepiota sp. extract gave favorable results in inhibiting the spore germination of C. albicans. The percentage of spore germination was reduced when the extract concentration was increased. Extract concentration has no significant effect on germ tube elongation.
| Item Type: | Academic Exercise |
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| Keyword: | Staphylococcus aureus, infection, Candida albicans, Lepiota sp. extract, temperature |
| Subjects: | Q Science > QK Botany |
| Department: | SCHOOL > School of Science and Technology |
| Depositing User: | SITI AZIZAH BINTI IDRIS - |
| Date Deposited: | 03 Sep 2013 10:43 |
| Last Modified: | 23 Oct 2017 16:05 |
| URI: | https://eprints.ums.edu.my/id/eprint/6854 |
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