Wong, Swee Yoong (2013) The potential of non-cultivated marine seaweeds as a source of prebiotic. Universiti Malaysia Sabah. (Unpublished)
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Abstract
The aim of study is to evaluate the prebiotic effect of Sabah seaweeds namely Sargassum polycytum (brown) and Caulerpa lentillifera (green). Hot-water was employed to extract the seaweed polysaccharides before subjected to acid hydrolysis with 1.38 M and 2.0 M of sulphuric acid for 2, 4 and 6 hours. Total sugar and reducing sugar content of the seaweed polysaccharides were determined by colorimetric method. Three different media (MRS Basal Medium, MRS and Tryptic Soy broth) were used to evaluate the effect of acid hydrolysed seaweed polysaccharides on the growth of probiotic, Lactobacillus plantarum. Extraction yield of the polysaccharides from Caulerpa lentillifera was significant higher (p<0.05) than Sargassum polycytum. Higher concentration of sulphuric acid (2.0 M) and longer duration hour of hydrolysis produced higher total sugar (0.83 mg/mL) and reducing sugar (0.55 mg/mL) content. Result showed that the growths of probiotic in MRS and MRS Basal Medium supplemented with acid hydrolysed Caulerpa lentillifera polysaccharides (2 h) are comparable to the inulin (standard). Similar results were obtained for acid hydrolysed polysaccharide from Sargassum polycytum, but the effect was inferior as compare to the inulin. It was found that MRS media supplemented with acid hydrolysed seaweed polysaccharides was more suitable for probiotic growth as the maximum viable count of probiotic reached 9 log CFU/mL as compare to 8 log CFU/mL for the other two media. The growth of probiotic in the media supplemented with 1.38 M and 2.0 M of acid hydrolysed seaweed extract was almost the same, although a higher concentration of acid (2.0 M) produced three-fold higher reducing sugars. These suggest the growth of probiotic was not depending on the present of high concentration of simple sugars but also the effect of partially acid hydrolysed seaweed polysaccharides. Overall, the qrowth of L. plantarum in all media supplemented with acid hydrolysed polysaccharides extract from Caulerpa lentillifera was significant higher (p<0.05) than the growth of the same probiotic in the medium supplemented with polysaccharides extract from Sargassum polycytum. In conclusion, acid hydrolysed polysaccharides extracted from Sargassum polycytum and Caulerpa lentillifera showed potential as a source of prebiotic due to their ability to promote growth of probiotic, Lactobacillus planfiJrum. However, further study should be carried out to evaluate the prebiotic activity of the selected add hydrolysed seaweed polysaccharides by in vivo method and determining the molecular weight of the acid hydrolysed polysaccharides.
| Item Type: | Academic Exercise |
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| Keyword: | Sargassum polycytum, Caulerpa lentillifera, in vivo method, Hot-water, probiotic |
| Subjects: | ?? TP368-456_Food_processing_and_manufacture ?? |
| Department: | SCHOOL > School of Food Science and Nutrition |
| Depositing User: | SITI AZIZAH BINTI IDRIS - |
| Date Deposited: | 13 Nov 2014 15:56 |
| Last Modified: | 13 Oct 2017 15:09 |
| URI: | https://eprints.ums.edu.my/id/eprint/9804 |
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